Stability of fatty acids during wilting of perennial ryegrass (Lolium perenne L.): effect of bruising and environmental conditions.
Stability of fatty acids during wilting of perennial ryegrass (Lolium perenne L.): effect of bruising and environmental conditions.
复制标题
多年生黑麦草（Lolium perenne L.）枯萎期间脂肪酸的稳定性：瘀伤和环境条件的影响。
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
W. Hendriks
中科院分区：
文献类型：
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作者：
N. Khan;J. Cone;V. Fievez;W. Hendriks
背景
牧草田间萎蔫过程中脂肪酸的氧化会造成多不饱和脂肪酸的大量损失。最近的研究表明，萎蔫对FA的损失有不同的影响。这表明环境和管理条件影响枯萎期间FA的损失。本研究调查了FA在未处理和机械损伤的多年生黑麦草中的稳定性，在田间条件下萎蔫0，12，24，36和48 h，或在受控气候条件下萎蔫，在三个温度（15，25或35 °C）和两个光照（黑暗或光照）制度下，干物质（DM）含量为425，525或625 g kg（-1）。
结果
在48小时的田间萎蔫，总FA含量下降（15.2至11.9克kg（-1）DM）一致，尽管牧草DM含量增加（197至676克kg（-1））。在受控气候条件下，牧草总FA含量下降（15.1至11.7 g kg（-1）DM）主要是在延长（56至62 h）的初始干燥至DM含量为425 g kg（-1），并没有随着进一步干燥至DM含量为525和625 g kg（-1）而下降。在田间（9.15至6.36 g kg（-1）DM）和对照（9.12至6.15 g kg（-1）DM）条件下，总FA的下降与C18：3含量的平行下降相关。同时，C18：3在总FA中的比例下降，而C16：0和C18：0的比例增加。与25和35 °C相比，在15 °C下观察到较低的FA损失（P<0.05）。光照不影响萎蔫过程中FA的损失。
结论
萎蔫期的长短主要影响FA含量和组成的变化。牧草中FA的稳定性可以通过最小化萎蔫的持续时间来增加。
BACKGROUND
Oxidation of fatty acids (FA) during field wilting of herbage could cause extensive losses of polyunsaturated FA. Recent studies showed a variable effect of wilting on the losses of FA. This suggests that environment and management conditions influence the loss of FA during wilting. The present study investigated the stability of FA in untreated and mechanically bruised perennial ryegrass, wilted under field conditions for 0, 12, 24, 36 and 48 h, or wilted under controlled climate conditions at three temperatures (15, 25 or 35 °C) and two light (dark or light) regimes to dry matter (DM) contents of 425, 525 or 625 g kg(-1).
RESULTS
During 48 h of field wilting, the total FA content declined (15.2 to 11.9 g kg(-1) DM) consistently, despite an increase in herbage DM content (197 to 676 g kg(-1)). Under controlled climate conditions, the herbage total FA content declined (15.1 to 11.7 g kg(-1) DM) mainly during the prolonged (56 to 62 h) initial drying to a DM content of 425 g kg(-1) and did not decline with further drying to DM contents of 525 and 625 g kg(-1). The decline in total FA was associated with a parallel decline in C18:3 content under field (9.15 to 6.36 g kg(-1) DM) and controlled (9.12 to 6.15 g kg(-1) DM) conditions. Concomitantly, the proportion of C18:3 in total FA decreased, whilst the proportion of C16:0 and C18:0 increased. Lower losses of FA (P<0.05) were observed at 15 °C compared to 25 and 35 °C. Light did not affect the losses of FA during wilting.
CONCLUSIONS
The duration of the wilting period mainly affected the changes in FA content and composition. Stability of FA in herbage could be increased by minimising the duration of wilting.