Optical silencing of C. elegans cells with light-driven proton pumps
Optical silencing of C. elegans cells with light-driven proton pumps
复制标题
用光驱动质子泵对秀丽隐杆线虫细胞进行光学沉默
DOI：
10.1016/j.ymeth.2014.02.030
复制
发表时间：
2014
期刊：
影响因子：
4.8
通讯作者：
Shin Takagi
中科院分区：
文献类型：
--
作者：
Ayako Okazaki;Megumi Takahashi;Naoya Toyoda;Shin Takagi
近年来，利用光驱动离子通道或离子泵控制可兴奋细胞活性的光遗传技术的发展，极大地促进了活体神经系统的研究。新一代光学消音器包括外向式质子泵，如Arch，它比目前广泛使用的卤代紫质(NpHR)有几个优点。这些优势包括在长时间光照下抵抗失活，以及在低强度光下产生更大的光学电流的能力。线虫具有较小的透明身体和特征良好的神经电路，特别适合于光遗传分析。在这篇文章中，我们将概述使用Arch和其他质子泵作为光遗传工具公司的实际方面。优雅女装。
Recent development of optogenetic techniques, which utilize light-driven ion channels or ion pumps for controlling the activity of excitable cells, has greatly facilitated the investigation of nervous systemsin vivo. A new generation of optical silencers includes outward-directed proton pumps, such as Arch, which have several advantages over currently widely used halorhodopsin (NpHR). These advantages include the resistance to inactivation during prolonged illumination and the ability to generate a larger optical current from low intensity light.C. elegans, with its small transparent body and well-characterized neural circuits, is especially suitable for optogenetic analyses. In this article, we will outline the practical aspects of using of Arch and other proton pumps as optogenetic tools inC. elegans.