Hindlimb Suspension Does Not Influence Mechanical Sensitivity, Epidermal Thickness, and Peripheral Nerve Density in the Glabrous Skin of the Rat Hind Paw
Hindlimb Suspension Does Not Influence Mechanical Sensitivity, Epidermal Thickness, and Peripheral Nerve Density in the Glabrous Skin of the Rat Hind Paw
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DOI：
10.33549/physiolres.932348
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发表时间：
2013-01-01
影响因子：
2.1
通讯作者：
Okita, M.
中科院分区：
文献类型：
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作者：
Tanaka, Y.;Nakano, J.;Okita, M.
我们的目的是在大鼠后肢悬浮 (HS) 模型中研究微重力对皮肤对机械刺激的敏感性、表皮厚度、真皮上层周围神经密度以及应激标记物血清水平的影响。 30只8周龄雄性Wistar大鼠随机分为3组：HS组，n=10；假HS，n=10；对照，n=10。 HS组和假HS组均将悬挂系统附着在大鼠尾部，但仅HS组的后肢悬挂。 HS组和假HS组治疗4周。在使用 von-Frey 细丝（每组 n=5）的行为测试中，HS 组和假 HS 组出现了机械超敏反应。与对照组相比，HS组和假HS组的血清皮质酮水平显着升高，并且在去除HS后立即观察到表皮厚度或周围神经密度没有变化（每组n = 5）。这些数据表明，在 HS 组中观察到的机械超敏反应不是由微重力或不活动引起的，而是由约束应力引起的。我们认为微重力不会影响这些动物的皮肤敏感性和组织学。
Our aim was to investigate the influence of microgravity on the sensitivity of the skin to mechanical stimulation, epidermal thickness, peripheral nerve density in the upper dermis, and serum levels of a stress marker in a rat hindlimb suspension (HS) model. Thirty 8-week-old male Wistar rats were randomly divided into 3 groups: HS, n=10; sham HS, n=10; control, n=10. The suspension system was attached to rat tails in both the HS and sham-HS groups, but the hindlimbs were suspended only in the HS group. The HS and sham-HS groups were treated for 4 weeks. In behavioral tests using von-Frey filaments (n=5 in each group), mechanical hypersensitivity developed in the HS and sham HS groups. Serum corticosterone levels increased significantly in the HS and sham HS groups compared to the control group, and no changes in epidermal thickness or peripheral nerve density were observed immediately after the removal of HS (n=5 in each group). These data indicated that the mechanical hypersensitivity observed in the HS group was not caused by microgravity or inactivity, but rather by restraint stress. We suggest that microgravity does not affect skin sensitivity and histology in these animals.