Heterogeneity in the neuropeptide Y-containing neurons of the rat arcuate nucleus: GABAergic and non-GABAergic subpopulations
Heterogeneity in the neuropeptide Y-containing neurons of the rat arcuate nucleus: GABAergic and non-GABAergic subpopulations
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DOI：
10.1016/s0006-8993(97)00184-4
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发表时间：
1997-05-09
期刊：
影响因子：
2.9
通讯作者：
Leranth, C
中科院分区：
文献类型：
--
作者：
Horvath, TL;Bechmann, I;Leranth, C
下丘脑弓状核产生的神经肽Y在垂体前叶和食欲功能的中枢调节中起关键作用。神经肽Y在这些机制中的多效性表明产生神经肽Y的下丘脑神经元群体中存在异质性。在这项研究中，我们报告的共存的神经肽Y和氨基酸递质，γ-氨基丁酸（GABA），在神经元胞体的弓状核。荧光双免疫标记的神经肽Y和谷氨酸脱羧酶进行振动切片收集通过下丘脑弓状核的动物，用秋水仙碱预处理。结果发现，约三分之一的神经肽Y产生的弓状核胞体共表达谷氨酸脱羧酶。这群含神经肽Y的GABA能神经元纵向分布在弓状核内，主要位于其背内侧。这些结果表明，在弓状核中至少有两个不同的神经肽Y产生神经元的群体：位于背内侧弓状核中的神经肽Y和GABA共同产生神经元的子集和位于腹侧弓状核中的非GABA能神经肽Y细胞的子集。下丘脑神经肽Y产生细胞核的这种异质性可能有助于解释弓状核神经肽Y的不良神经内分泌和行为效应。
Neuropeptide Y, produced in the arcuate nucleus of the hypothalamus, plays a key role in the central regulation of anterior pituitary and appetitive functions. The pleiotropic nature of neuropeptide Y in these mechanisms indicates the existence of heterogeneity in the hypothalamic neuronal population producing neuropeptide Y. In this study, we report the coexistence of neuropeptide Y and the amino acid transmitter, gamma-aminobutyric acid (GABA), in neuronal perikarya of the arcuate nucleus. Fluorescent double immunolabeling for neuropeptide Y and glutamic acid decarboxylase was carried out on vibratome sections collected through the hypothalamic arcuate nuclei of animals that were pretreated with colchicine. It was found that about one third of the neuropeptide Y-producing arcuate nucleus perikarya co-expressed glutamic acid decarboxylase. This population of neuropeptide Y-containing GABAergic neurons were distributed longitudinally within the arcuate nucleus located predominantly in its dorsomedial aspects. These results show that there are at least two distinct populations of neuropeptide Y-producing neurons in the arcuate nucleus: a subset of neuropeptide Y and GABA-co-producing neurons located in the dorsomedial arcuate nucleus and a subset of non-GABAergic neuropeptide Y cells located in the ventral arcuate nucleus. This heterogeneity in the neuropeptide Y-producing perikarya of the hypothalamus may help explain adverse neuroendocrine and behavioral effects of arcuate nucleus neuropeptide Y.