EXPRESSION OF SMOOTH MUSCLE-SPECIFIC PROTEINS IN MYOEPITHELIUM AND STROMAL MYOFIBROBLASTS OF NORMAL AND MALIGNANT HUMAN BREAST-TISSUE
EXPRESSION OF SMOOTH MUSCLE-SPECIFIC PROTEINS IN MYOEPITHELIUM AND STROMAL MYOFIBROBLASTS OF NORMAL AND MALIGNANT HUMAN BREAST-TISSUE
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DOI：
10.1073/pnas.90.3.999
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发表时间：
1993-02-01
影响因子：
11.1
通讯作者：
KOTELIANSKY, VE
中科院分区：
文献类型：
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作者：
LAZARD, D;SASTRE, X;KOTELIANSKY, VE
通过冷冻切片的免疫荧光显微镜研究了正常人乳腺肌上皮和与乳腺癌相关的某些基质成纤维细胞（“肌成纤维细胞”）中几种分化标志物的表达。使用针对平滑肌特异性蛋白（平滑肌α肌动蛋白、平滑肌肌球蛋白重链、钙调蛋白、α1-整合素和高分子量钙结合蛋白）和上皮特异性蛋白（细胞角蛋白、E-钙粘蛋白和桥粒斑蛋白）的几种抗体来显示肌上皮细胞同时表达上皮和平滑肌标记物，而相邻的管腔细胞仅表达上皮标记物。使用相同的抗体来确定基质肌成纤维细胞表现出平滑肌表型特性，其特征在于除了高分子量卡尔德斯蒙之外所检查的所有平滑肌标记物的表达。此外，肌上皮和肌成纤维细胞在平滑肌蛋白表达方面均表现出显着程度的异质性。因此，肌上皮细胞和基质肌成纤维细胞分别是上皮细胞和间充质细胞，它们协调表达一组平滑肌标记物，同时保持其特定的原始特征。肌上皮细胞的双重性质和成纤维细胞向肌成纤维细胞的表型转变是分化细胞表型可塑性的例子。
The expression of several differentiation markers in normal human mammary gland myoepithelium and in certain stromal fibroblasts (''myofibroblasts'') associated with breast carcinomas was studied by immunofluorescence microscopy of frozen sections. Several antibodies to smooth muscle-specific proteins (smooth muscle alpha-actin, smooth muscle myosin heavy chains, calponin, alpha1-integrin, and high molecular weight caldesmon) and to epithelial-specific proteins (cytokeratins, E-cadherin, and desmoplakin) were used to show that myoepithelial cells concomitantly express epithelial and smooth muscle markers whereas adjacent luminal cells express only epithelial markers. The same antibodies were used to establish that stromal myofibroblasts exhibit smooth muscle phenotypic properties characterized by the expression of all the smooth muscle markers examined except for high molecular weight caldesmon. In addition, both myoepithelium and myofibroblasts show a significant degree of heterogeneity in smooth muscle protein expression. Thus, myoepithelial cells and stromal myofibroblasts are epithelial and mesenchymal cells, respectively, which coordinately express a set of smooth muscle markers while maintaining their specific original features. The dual nature of myoepithelial cells and the phenotypic transition of fibroblasts to myofibroblasts are examples of the plasticity of the differentiated cell phenotype.