Embryonal Carcinoma and Glioblastoma Cell Lines Derived from Monkey Induced Pluripotent Stem Cells
Embryonal Carcinoma and Glioblastoma Cell Lines Derived from Monkey Induced Pluripotent Stem Cells
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源自猴诱导多能干细胞的胚胎癌和胶质母细胞瘤细胞系
DOI：
10.1007/7651_2020_303
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发表时间：
2020
影响因子：
--
通讯作者：
Itoh Yasushi
中科院分区：
文献类型：
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作者：
Ishigaki Hirohito;Itoh Yasushi
诱导多能干细胞 (iPSC) 可用于再生医学疗法的开发、发病机制分析和候选药物的探索。我们开发了 iPSC 的另一种用途，其中 MHC 单倍型与食蟹猴模型中的可移植受体相匹配。我们从食蟹猴 iPSC 中建立了两种癌细胞系：胚胎癌和胶质母细胞瘤细胞系。在这里，我们描述了一种诱导癌细胞系的方法，包括在饲养细胞上培养猴 iPSC 的技术以及从猴 iPSC 诱导造血干细胞和神经祖细胞的技术。
Induced pluripotent stem cells (iPSCs) are useful for the development of therapies in regenerative medicine, analysis of pathogenesis, and exploration of candidate drugs. We developed an alternative usage of iPSCs of which the MHC haplotype is matched to transplantable recipients in a cynomolgus macaque model. We established two cancer cell lines, embryonal carcinoma, and glioblastoma cell lines from cynomolgus monkey iPSCs. Here, we describe a method to induce the cancer cell lines including a technique for culture of the monkey iPSCs on feeder cells and the induction of hematopoietic stem cells and neural progenitor cells from monkey iPSCs.