Mammaglobin B as a novel marker for detection of breast cancer micrometastases in axillary lymph nodes by reverse transcription-polymerase chain reaction
Mammaglobin B as a novel marker for detection of breast cancer micrometastases in axillary lymph nodes by reverse transcription-polymerase chain reaction
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DOI：
10.1023/a:1006335817889
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发表时间：
1999-11-01
影响因子：
3.8
通讯作者：
Monden, M
中科院分区：
文献类型：
--
作者：
Aihara, T;Fujiwara, Y;Monden, M
建立了一种新的乳腺珠蛋白B基因逆转录-聚合酶链反应（RT-PCR）检测乳腺癌腋窝淋巴结微转移的方法。对14个原发性乳腺癌和来自6个原发性乳腺癌患者的56个腋窝淋巴结以及来自非癌症携带患者的15个对照淋巴结进行该测定。乳腺珠蛋白B基因转录本在对照淋巴结中均未检测到，但在14例原发性乳腺癌中均检测到。在组织学检查显示阳性的患者的56个淋巴结中，有11个淋巴结也被该测定证明为阳性。另一方面，45个组织学阴性的淋巴结中有14个（31%）也显示表达乳房珠蛋白B mRNA，这表明这些淋巴结中存在微转移。因此，使用乳房珠蛋白B基因的RT-PCR可能成为检测乳腺癌微转移的有用工具。
A novel reverse transcription-polymerase chain reaction (RT-PCR) assay using mammaglobin B gene was developed for detection of breast cancer micrometastases in axillary lymph nodes. Fourteen primary breast cancers and 56 axillary lymph nodes from six patients with primary breast cancer and 15 control lymph nodes from non-cancer bearing patients were subjected to this assay. The transcript of mammaglobin B gene was detected in none of the control lymph nodes, but in all of the 14 primary breast cancers. Eleven out of the 56 lymph nodes from the patients, which were shown to be positive by histological examination, were also proven positive by this assay. On the other hand, fourteen of the 45 (31%) histologically negative lymph nodes were also shown to express mammaglobin B mRNA, which suggested the presence of micrometastases in these lymph nodes. RT-PCR using mammaglobin B gene could therefore be a useful tool for detection of micrometastases of breast cancer.