Bone marrow stromal cells act as feeder cells for tendon fibroblasts through soluble factors
Bone marrow stromal cells act as feeder cells for tendon fibroblasts through soluble factors
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DOI：
10.1089/ten.2006.0079
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发表时间：
2007-02-01
影响因子：
--
通讯作者：
Minami, Akio
中科院分区：
文献类型：
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作者：
Shimode, Kazumi;Iwasaki, Norimasa;Minami, Akio
采用共培养的方法，研究了骨髓基质细胞（BMSCs）对肌腱成纤维细胞的饲养效应，为肌腱组织工程和细胞治疗提供实验依据。BMSCs对增强细胞增殖、细胞迁移能力和细胞粘附性有显著作用，但对无细胞-细胞接触的肌腱成纤维细胞的细胞外基质（ECM）合成影响不大。此外，尽管存在成纤维细胞，但来自BMSC的条件培养基显著增加了成纤维细胞的数量。根据这些结果，饲养层效应的机制被认为是从BMSCs到成纤维细胞的可溶性因子的某种信号。比较蛋白质组学分析的条件培养基从共培养的成纤维细胞和骨髓基质细胞显示较少的纤溶酶原的表达，这表明对成纤维细胞增殖的抑制作用。关于纤溶酶原和BMSCs在共培养体系中的关系，我们推测BMSCs通过某种机制使纤溶酶原或其裂解活性在培养基中分解。
Feeder effects of bone marrow stromal cells (BMSCs) on tendon fibroblasts were investigated using a coculture method for the application of ligament or tendon tissue engineering and cell therapy. BMSCs had significant effects on enhancing cell proliferation, the ability of cells to migrate, and cell adhesivity but little effect on the extracellular matrix (ECM) synthesis of tendon fibroblasts without cell-cell contact. Furthermore, the conditioned medium from BMSCs, despite the existence of fibroblasts, significantly increased the number of fibroblasts. Based on these results, the mechanism of the feeder effects is considered to be a certain signal of soluble factors from BMSCs to the fibroblasts. Comparative proteome analysis of the conditioned medium from co-culture of fibroblasts and BMSCs revealed less expression of plasminogen, which showed inhibitory effects on fibroblast proliferation. With regard to the relationships between plasminogen and BMSCs in the co-culture system, we speculate that BMSCs allow resolution of plasminogen or its cleavage activity in the medium via some mechanism.