An ordered addition, essential activation model of the tissue factor pathway of coagulation: evidence for a conformational cage.
An ordered addition, essential activation model of the tissue factor pathway of coagulation: evidence for a conformational cage.
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凝血组织因子途径的有序添加、基本激活模型：构象笼的证据。
DOI：
10.1021/bi00362a006
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Gentry,R
中科院分区：
文献类型：
--
作者：
Nemerson,Y;Gentry,R
材料与方法制备鼠抗牛脑组织因子单克隆抗体。卡森（现就职于科罗拉多大学）和R.我们小组的巴赫（卡森等人，1985).制备牛因子VII、IX和X的基本上均质的制剂，并如前所述进行定量（Bach等人，1984; Kalousek等人，1975; Jesty & Nemerson，1976）.如前所述，通过用因子Xa裂解因子VII来制备因子Vila（Radcliffe & Nemerson，1975）。通过还原样品的电泳判断，所得产物为双链形式（Laemmli，1970）。该酶具有酶原的100-120倍的凝血活性（Bach等人，1984年）。组织因子是一种膜结合糖蛋白，在纯化过程中被脱脂，在这种状态下它是无活性的。如前所述纯化的脱辅基蛋白（Bach等人，1981），通过掺入由指定比例的磷脂酰胆碱（卵）和磷脂酰丝氨酸（牛）组成的囊泡中而被再活化。除非另有说明，否则用纯磷脂酰胆碱完成再脂质化。脂质获自Supelco，Inc.，宾夕法尼亚州贝莱丰特。采用辛基葡萄糖苷法进行了复溶
Materials and MethodsThe murine monoclonal antibody to bovine brain tissue factor was prepared and characterized by S. Carson (presently at the University of Colorado) and R. Bach of our group (Carson et al., 1985).Essentially homogeneous preparations of bovine factors VII, IX, and X were prepared and quantified as previously de-scribed (Bach et al., 1984; Kalousek et al., 1975; Jesty & Nemerson, 1976). Factor Vila was prepared by cleaving factor VII with factor Xa as previously described (Radcliffe & Nemerson, 1975). The resultant product was in the two-chain form as judged by electrophoresis of a reduced sample (Laemmli, 1970). The enzyme possessed 100-120 times the coagulant activityof the zymogen (Bach et al., 1984). Tissue factor, a membrane-bound glycoprotein, is delipidated during purification, in which state it is inactive. The apo-protein, which was purified as previously described (Bach et al., 1981), was reactivated by incorporation into vesicles consisting of phosphatidylcholine (egg) and phosphatidylserine (bovine) at the indicated ratios. Unless stated, relipidation was accomplished with pure phosphatidylcholine. The lipids were obtained from Supelco, Inc., Bellefonte, PA. Recon-stitution was accomplished by using the octyl glucoside method
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人皮肤成纤维细胞胶原酶抑制剂。
DOI：
10.1016/s0021-9258(17)44166-4
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Stricklin;H. Welgus
通讯作者：
H. Welgus
DOI：
10.1016/s0021-9258(17)37747-5
发表时间：
1979-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
H. Welgus;G. Stricklin;A. Eisen;E. Bauer;R. Cooney;J. Jeffrey
通讯作者：
H. Welgus;G. Stricklin;A. Eisen;E. Bauer;R. Cooney;J. Jeffrey
DOI：
10.1016/s0021-9258(18)34459-4
发表时间：
1982-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Castagna;Y. Takai;K. Kaibuchi;K. Sano;U. Kikkawa;Y. Nishizuka
通讯作者：
M. Castagna;Y. Takai;K. Kaibuchi;K. Sano;U. Kikkawa;Y. Nishizuka
人成纤维细胞胶原酶抑制剂的一级结构和cDNA克隆。
DOI：
10.1073/pnas.83.8.2407
发表时间：
1986
影响因子：
11.1
作者：
D. Carmichael;A. Sommer;R. Thompson;David C. Anderson;Christopher G. Smith;Howard G. WELGUSt;G. Stricklin
通讯作者：
G. Stricklin
培养中的人成纤维细胞生物合成金属蛋白酶组织抑制剂。
影响因子：
4.8
作者：
G. Murphy;J. Reynolds;Z. Werb
通讯作者：
Z. Werb