GOOSE PARVOVIRUS - AN AUTONOMOUS MEMBER OF THE DEPENDOVIRUS GENUS
GOOSE PARVOVIRUS - AN AUTONOMOUS MEMBER OF THE DEPENDOVIRUS GENUS
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DOI：
10.1006/viro.1995.1345
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发表时间：
1995-07-10
期刊：
影响因子：
3.7
通讯作者：
YOUNG, NS
中科院分区：
文献类型：
--
作者：
BROWN, KE;GREEN, SW;YOUNG, NS
鹅细小病毒是德兹病（一种幼鹅致命性肝炎）的病原体。该病毒感染鹅和番鸭，并且可以在实验室的初级胚胎鹅成纤维细胞中繁殖。迄今为止，该病毒仅根据形态、生化和培养特征被归类为自主细小病毒。我们现在报告病毒基因组 3434 个核苷酸的克隆和部分测序。获得了三个重叠克隆，编码非结构区和衣壳编码区。该核苷酸序列与其他自主细小病毒几乎没有同源性，但与依赖病毒 AAV2 有 55% 同源性。与 AAV2 的这种同源性也在氨基酸水平上得到了证实（非结构蛋白 55%，衣壳编码区 51%）。 DNA 交叉杂交研究表明，鹅细小病毒与尚未测序的人类依赖病毒 AAV1 和 AAV3 的相似性比 AAV2 更接近。这些发现表明，鹅细小病毒可能与依赖病毒属而不是其他自主细小病毒在遗传上相关。
Goose parvovirus is the etiological agent of Derzsy's disease, a fatal hepatitis of young geese. The Virus infects geese and Muscovy ducks and can be propagated in the laboratory in primary embryonic goose fibroblasts. To date the virus has only been classified by morphological, biochemical, and culture characteristics as an autonomous parvovirus. We now report the cloning and partial sequencing of 3434 nucleotides of the viral genome. Three overlapping clones were obtained, encoding regions in the nonstructural and capsid coding region. The nucleotide sequence show little homology to other autonomous parvoviruses but 55% homology to the dependovirus AAV2. This homology to AAV2 was also confirmed at the amino acid level (nonstructural protein 55%, capsid coding region 51%). DNA cross hybridization studies indicate an even closer similarity of goose parvovirus to the yet unsequenced human dependoviruses AAV1 and AAV3 than to AAV2. These findings suggest that goose parvovirus may be genetically related to the dependovirus genus rather than to the other autonomous parvoviruses.