Immunization of rabbits with recombinant serine protease inhibitor reduces the performance of adult female Rhipicephalus microplus
Immunization of rabbits with recombinant serine protease inhibitor reduces the performance of adult female Rhipicephalus microplus
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DOI：
10.1111/j.1865-1682.2010.01108.x
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发表时间：
2010-04-01
影响因子：
4.3
通讯作者：
Stich, R. W.
中科院分区：
文献类型：
--
作者：
Jittapalapong, S.;Kaewhom, P.;Stich, R. W.
蜱唾液腺分泌的分子调节脊椎动物宿主的免疫反应，因此代表了新的蜱控制措施的潜在目标。蜱唾液腺丝氨酸蛋白酶抑制剂（Serpin）是一种这样的分子，其可以促进蜱进食、血粉消化和病原体传播。研究重组微小扇头蜱唾液腺丝氨酸蛋白酶抑制剂（rSerpin）对家兔的免疫原性和保护作用。用rSerpin、佐剂或单独的磷酸盐缓冲盐水（PBS）注射家兔，并用500 R攻击感染。microplus幼虫，使其在成虫阶段连续进食和蜕皮。免疫后第2周所有兔均产生抗rSerpin抗体。与PBS对照组相比，用免疫兔喂养的蜱虫导致成虫饱胀减少83%，卵团重量减少34%。这些结果表明这种蜱Serpin对兔具有免疫原性，并表明这种疫苗候选抗原可以在该实验模型中赋予针对牛蜱的保护性免疫。
P>Molecules secreted from the tick salivary gland modulate the vertebrate host immune response, thus representing potential targets for novel tick control measures. Tick salivary gland serine protease inhibitor (Serpin) is one such molecule that may facilitate tick feeding, blood meal digestion and pathogen transmission. The objective of this study was to determine the immunogenicity and protection of recombinant Rhipicephalus (Boophilus) microplus salivary gland Serpin (rSerpin) in rabbits. Rabbits were injected with rSerpin, adjuvant or phosphate-buffered saline (PBS) alone, and challenge infested with 500 R. microplus larvae that were allowed to continuously feed and moult through the adult stage. All immunized rabbits generated antibodies to rSerpin in the second week after immunization. Ticks fed on immunized rabbits resulted in 83% reduction in adult engorgement and 34% reduction in egg mass weight compared with the PBS control. These results indicated that this tick Serpin is immunogenic to rabbits, and suggested that this vaccine candidate antigen can confer protective immunity against cattle ticks in this experimental model.