The effect of microcomputed tomography scanning and reconstruction voxel size on the accuracy of stereological measurements in human cancellous bone
The effect of microcomputed tomography scanning and reconstruction voxel size on the accuracy of stereological measurements in human cancellous bone
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DOI：
10.1016/j.bone.2004.09.007
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发表时间：
2004-12-01
期刊：
影响因子：
4.1
通讯作者：
Yeni, YN
中科院分区：
文献类型：
--
作者：
Kim, DG;Christopherson, GT;Yeni, YN
体视学参数已被用作骨小梁结构的近似值。骨体积分数(Bv/Tv)、骨小梁数目(Tb.N)、骨小梁厚度(Tb.Th)、骨小梁间距(Tb.Sp)、骨表面积体积比(BS/BV)、各向异性程度(MIL1/MIL3)和连接密度(-Euler/Vol)等结构性指标已被广泛研究用于研究骨的病理状态。由于其高分辨率和非破坏性，微计算机断层成像(Micro-CT)被用来获取小梁微结构的精确三维(3D)图像。在这项研究中，三种不同的扫描和重建体素大小的组合被用来表示最佳的体素大小(21微米；为了检查扫描和重建体素大小对人体松质骨立体学测量的影响，相对于本研究中使用的其他体素大小，常用的中间体素大小(50微米)和适用于整个人体椎体扫描的中等体素大小(110微米)。使用大体素大小的组合计算的体素参数相对于黄金标准(最佳情况)的误差在0.1%到102%之间。在其他情况下，相对于金标准的误差的符号大小是扫描或重建体素大小或两者的函数(r(2)=0.55-0.95)。对于大多数结构指数，具有较大体素大小的图像的分析结果与金标准的结果相关(r(2)=0.55-0.99)，除了Tb.N在110/110微米，MIL1/MIL3在大于110微米的重建体素大小，以及-Euler/Vol在任何体素大小的组合。总体而言，观察到在较低分辨率(对应于在本研究中增加重建体素大小)下对高分辨率图像进行重采样与在相同低分辨率(对应于在本研究中增加扫描体素大小)下扫描对计算参数的影响不同。我们的结果表明，对图像分辨率的研究应该包括以感兴趣的分辨率进行的实际扫描，而不是像通常所做的那样简单地对高分辨率图像进行粗化。(C)2004 Elsevier Inc.保留所有权利。
Stereological parameters have been used as an approximation for the architecture of trabecular bone. Structural indices such as bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), bone surface-to-volume ratio (BS/BV), degree of anisotropy (MIL1/MIL3), and connectivity density (-Euler/Vol) have been widely studied to investigate pathological conditions in bone. Due to its high resolution and nondestructiveness, microcomputed tomography (micro-CT) has been utilized to take precise three-dimensional (3D) images of trabecular microstructures. However, spatial limitations for applying micro-CT-based analyses to large specimens, such as whole vertebral bodies, require using larger scanning and reconstruction voxel sizes.In this study, combinations of three different scanning and reconstruction voxel size were used to represent best possible voxel size (2 1 mum; best in our scanner for the specimen size used) relative to other voxel sizes used in this study, commonly used intermediate voxel sizes (50 mum), and those applicable to scans of whole human vertebral bodies (110 mum) in order to examine the effect of scanning and reconstruction voxel size on stereological measures for human cancellous bone.The error in stereological parameters calculated using combinations of large voxel sizes compared to the gold standard (best possible case) ranged from 0.1% to 102%. The signed magnitude of the error in other cases relative to the gold standard was a function of either scanning or reconstruction voxel size or both (r(2) = 0.55-0.95). For most of the structural indices, the results from analysis of images with larger voxel sizes were correlated with those from the gold standard (r(2) = 0.55-0.99) except for Tb.N at 110/110 mum, MIL1/MIL3 at larger than 110 mum reconstruction voxel size, and -Euler/Vol at any combination of voxel sizes. Overall, it was observed that resampling a high resolution image at lower resolutions (corresponding to increasing reconstruction voxel size in this study) had different effects on the Calculated parameters than scanning at the same low resolution (corresponding to increasing scanning voxel size in this study). Our results show that investigations of image resolution should include actual scans at the resolution of interest rather than simply coarsening of high-resolution images as is customarily done. (C) 2004 Elsevier Inc. All rights reserved.