Aquatic Bird Bornavirus-Associated Disease in Free-Living Canada Geese (Branta canadensis) in the Northeastern USA
Aquatic Bird Bornavirus-Associated Disease in Free-Living Canada Geese (Branta canadensis) in the Northeastern USA
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DOI：
10.7589/2016-07-160
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发表时间：
2017-07-01
影响因子：
1.3
通讯作者：
O'Brien, Kathleen M.
中科院分区：
文献类型：
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作者：
Murray, Maureen;Guo, Jianhua;O'Brien, Kathleen M.
2013-14 年冬季，22 只加拿大鹅 (Branta canadensis) 因非特异性神经系统异常和消瘦被送入塔夫茨大学卡明斯兽医学院野生动物诊所。通过组织病理学、免疫组织化学和逆转录 PCR (RT-PCR) 对其中五只鹅以及三只被提交死亡的鹅进行了博纳病毒的评估。组织病理学上，八只鸟中有六只患有淋巴浆细胞性脑炎。一只也患有脑炎的鸟食道扩张。诊断排除了铅中毒、西尼罗河病毒、禽流感和禽副粘病毒感染。免疫组织化学显示，所有八只鹅的脑组织均呈冰龙病毒 N 抗原阳性。五只鸟的冷冻脑组织可用于博纳病毒 RTPCR。五只鸟中的三只对博尔纳病毒 M 基因呈阳性。通过 RT-PCR 对其余三只鹅的福尔马林固定石蜡包埋的脑组织进行了评估，其中一只鹅检测结果呈阳性。随后在来自一只加拿大鹅大脑的鸭胚胎成纤维细胞中培养了一种博纳病毒。该病毒基因组已被测序，该病毒被鉴定为水禽博纳病毒1型。鉴于博纳病毒在水禽中的亚临床感染在北美很常见，我们无法识别该基因组的任何异常特征来解释其明显的致病性。
During the winter of 2013-14, 22 Canada geese (Branta canadensis) were admitted to the Wildlife Clinic at the Cummings School of Veterinary Medicine at Tufts University with nonspecific neurologic abnormalities and emaciation. Five of these geese, along with three geese that were submitted dead, were evaluated via histopathology, immunohistochemistry, and reverse transcription PCR (RT-PCR) for bornaviruses. Histopathologically, six of the eight birds had lymphoplasmacytic encephalitis. One bird, which also had encephalitis, had a dilated esophagus. Lead poisoning, West Nile virus, avian influenza, and avian paramyxovirus infection were excluded from the diagnosis. Brain tissue from all eight geese was positive for bornaviral N-antigen on immunohistochemistry. Frozen brain tissue from five birds was available for bornavirus RTPCR. Three of the five birds were positive for the bornavirus M gene. Formalin-fixed paraffin-embedded brain tissue was evaluated on the remaining three geese via RT-PCR, with one of these geese testing positive. A bornavirus was subsequently cultured in duck embryo fibroblasts from the brain of one Canada Goose. This virus genome was sequenced, and the virus was identified as aquatic bird bornavirus 1. We were unable to identify any unusual features of this genome that would account for its apparent pathogenicity, given that subclinical infection with bornavirus in waterfowl is common in North America.