Multiple rpoD-related genes of cyanobacteria.
Multiple rpoD-related genes of cyanobacteria.
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蓝藻的多个 rpoD 相关基因。
DOI：
10.1271/bbb.56.1113
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hideo Takahashi
中科院分区：
文献类型：
--
作者：
Kan Tanaka;Susumu Masuda;Hideo Takahashi
许多真细菌菌株的基因组已被证明编码多个rpoD相关基因。在这份报告中，我们描述了多个rpoD相关基因的蓝藻菌株的鉴定。利用合成的rpoD或rpoD相关基因探针，对鱼腥藻7120、聚球藻7942和集胞藻6803的DNA进行Southern杂交检测。在每个DNA中发现四个或五个杂交信号。克隆了聚球藻7942与杂交信号相对应的4个DNA片段，并进行了部分序列测定。序列数据表明存在名为rpoD1、rpoD2、rpoD3和rpoD4的基因，其产物与真细菌菌株的主要σ因子的基本结构高度相似。rpoD1基因与鱼腥藻7120的sigA基因相似性最高。
Genomes of many eubacterial strains have been shown to encode for multiple rpoD-related genes. In this report, we describe the identification of the multiple rpoD-related genes of cyanobacterial strains. DNAs of three cyanobacterial strains, Anabaena sp. PCC7120, Synechococcus sp. PCC7942, and Synechocystis sp. PCC6803, were examined by Southern hybridization, using a synthetic probe designed for detecting rpoD or rpoD-related genes. Four or five hybridization signals were found in each DNA. Four DNA regions of Synechococcus sp. PCC7942 corresponding to the hybridization signals were cloned and partially sequenced. The sequence data indicate the presence of genes, named rpoD1, rpoD2, rpoD3, and rpoD4, whose products are highly similar to the basic structure of the principal sigma factors of eubacterial strains. The rpoD1 gene showed the greatest similarity to the sigA gene of Anabaena sp. PCC7120.