High-temperature-induced transposition of insertion elements in Burkholderia multivorans ATCC 17616
High-temperature-induced transposition of insertion elements in Burkholderia multivorans ATCC 17616
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DOI：
10.1128/aem.71.4.1822-1828.2005
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发表时间：
2005-04-01
影响因子：
4.4
通讯作者：
Tsuda, M
中科院分区：
文献类型：
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作者：
Ohtsubo, Y;Genka, H;Tsuda, M
建立了一种高效、定量的分析多食伯克霍尔德氏菌ATCC 17616中各种插入序列(IS)转座的方法。将携带枯草芽孢杆菌sacB基因的质粒pGEN500导入ATCC 17616细胞，发现25%的耐蔗糖衍生物在pGEN500上携带各种IS元件。一种基于PCR的实验方法，其中使用了几个特定引物对的混合，揭示了pGEN500除了捕获了以前报道的五个IS元件(IS401、IS402、IS406、IS407和IS408)外，还捕获了三个新的IS元件，ISBmu1、ISBmu2和ISBmu3。这些IS元件的全球转位频率在高温条件下(42摄氏度)增加了七倍以上，但在氧化应激或饥饿条件下没有增加。据我们所知，这是第一个证明几种IS元素在高温下升高的转座活性的报告。本研究开发的高效实验方案将有助于定量和同时研究各种IS元素，以及从各种细菌中捕获新的功能活动元素。
An efficient and quantitative method to analyze the transposition of various insertion sequence (IS) elements in Burkholderia multivorans ATCC 17616 was devised. pGEN500, a plasmid carrying a Bacillus subtilis-derived sacB gene, was introduced into ATCC 17616 cells, and 25% of their sucrose-resistant derivatives were found to carry various IS elements on pGEN500. A PCR-based experimental protocol, in which a mixture of several specific primer pairs was used, revealed that pGEN500 captured, in addition to five previously reported IS elements (IS401, IS402, IS406, IS407, and IS408), three novel IS elements, ISBmu1, ISBmu2, and ISBmu3. The global transposition frequency of these IS elements was enhanced more than sevenfold under a high-temperature condition (42 degrees C) but not under oxidative stress or starvation conditions. To our knowledge, this is the first report demonstrating the elevated transposition activities of several IS elements at a high temperature. The efficient experimental protocol developed in this study will be useful in quantitatively and simultaneously investigating various IS elements, as well as in capturing novel functional mobile elements from a wide variety of bacteria.