Identification of neutralizing linear epitopes from the VP1 capsid protein of Enterovirus 71 using synthetic peptides
Identification of neutralizing linear epitopes from the VP1 capsid protein of Enterovirus 71 using synthetic peptides
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DOI：
10.1016/j.virusres.2006.12.005
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发表时间：
2007-04-01
期刊：
影响因子：
5
通讯作者：
Poh, Chit Laa
中科院分区：
文献类型：
--
作者：
Guang, Damian;Foo, Wei;Poh, Chit Laa
肠道病毒71型（EV 71）是手足口病（HFMD）的主要病原体，并与导致高死亡率的严重神经系统疾病有关。目前，没有可用的疫苗，治疗仅限于姑息治疗。在这项研究中，在小鼠中产生了针对跨越EV 71的VP 1衣壳蛋白的95个重叠合成肽的抗血清。两种分别含有VP 1的氨基酸163-177和208-222的肽SP 55和SP 70能够在体外微量中和试验中引发针对EV 71的中和抗体。SP 70被鉴定为在引发中和抗体滴度方面特别有效，所述中和抗体滴度与用全病毒体免疫血清获得的中和抗体滴度相当。用SP 55或SP 70免疫小鼠引发的EV 71特异性IgG应答与用完整病毒体作为免疫原获得的应答一样高。IgG 1亚型分型显示，由两种合成肽引起的中和抗体可能属于IgG 1亚型。与数据库比对结果表明，SP 70的氨基酸残基在EV 71各亚群毒株的VP 1序列中高度保守。总之，这些数据表明，SP 70代表了一个有希望的候选人的有效的合成肽为基础的疫苗，针对EV 71。(c)2007 Elsevier B. V.保留所有权利。
Enterovirus 71 (EV71) is the main causative agent of Hand, foot and mouth disease (HFMD) and has been associated with severe neurological diseases resulting in high mortalities. Currently, there is no vaccine available and treatment is limited to palliative care. In this study, antisera were raised in mice against 95 overlapping a synthetic peptides spanning the VP1 capsid protein of EV71. Two peptides, SP55 and SP70, containing amino acid 163-177 and 208-222 of VP1, respectively, are capable of eliciting neutralizing antibodies against EV71 in the in vitro microneutralization assay. SP70 was identified to be particularly potent in eliciting a neutralizing antibody titer comparable to that obtained with a whole virion-immune serum. Immunization of mice with either SP55 or SP70 triggered an EV71-specific IgG response as high as that obtained with the whole virion as immunogen. The IgG1 sub-typing revealed that the neutralizing antibodies elicited by both synthetic peptides are likely belonging to the IgG1 sub-type. Alignment with databases showed that the amino acid residues of SP70 are highly conserved amongst the VP1 sequences of EV71 strains from various sub-genogroups. Altogether, these data indicate that SP70 represents a promising candidate for an effective synthetic peptide-based vaccine against EV71. (c) 2007 Elsevier B.V. All rights reserved.