Effect of Nourishing "Yin" Removing "Fire" Chinese Herbal Mixture on Hypothalamic Mammalian Target of Rapamycin Expression during Onset of Puberty in Female Rats.
Effect of Nourishing "Yin" Removing "Fire" Chinese Herbal Mixture on Hypothalamic Mammalian Target of Rapamycin Expression during Onset of Puberty in Female Rats.
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DOI：
10.1155/2015/157846
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Tian Z
中科院分区：
文献类型：
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作者：
Zeng G;Han X;Yu J;Wang Y;Tian Z
瞄准本研究旨在探讨滋阴去火中药合剂对雌性大鼠青春期启动及下丘脑mTOR表达的影响。材料和方法。将40只20日龄雌性SD大鼠随机分为中药合剂组和生理盐水组。CHM组和NS组大鼠分别从第22天开始给予NYRF合剂和生理盐水。在d28、d31和d34处死各组大鼠。ELISA法检测血清促黄体生成素（LH）、促卵泡激素（FSH）和雌二醇（E2）水平。RT-PCR检测下丘脑mTOR mRNA表达水平，Western blot检测下丘脑p-mTOR蛋白水平。结果中药组阴道开放时间明显延迟（P < 0.05）。与NS组比较，中药组大鼠子宫系数、卵巢系数、血清LH、E2水平、下丘脑mTOR mRNA和p-mTOR蛋白表达水平均显著降低（P < 0.05）。结论滋阴去火中药合剂延缓青春期的发生可能与抑制下丘脑mTOR信号通路有关。
Aim. The present study aims to investigate the effects of nourishing “Yin” removing “Fire” (NYRF) Chinese herbal mixture on puberty onset and hypothalamic mTOR expression in female rats. Materials and Methods. Forty female 20-day-old Sprague-Dawley rats were randomly divided into Chinese herbal mixture (CHM) and normal saline (NS) groups. Rats in CHM and NS were treated with NYRF mixture and normal saline, respectively, from d22. Rats in each group were sacrificed on d28, d31, and d34. Serum luteinizing hormone (LH), follicle stimulating hormone (FSH), and estradiol (E2) levels were analyzed by ELISA. Hypothalamic mTOR mRNA expression levels were determined by RT-PCR and hypothalamic p-mTOR protein levels were assayed by western blot. Results. The vaginal opening time in CHM group was significantly delayed (P < 0.05). On d31, in comparison with NS group, the coefficients of uteri and ovaries, levels of serum LH and E2, and the expression levels of hypothalamic mTOR mRNA and p-mTOR protein were significantly lower in CHM group (P < 0.05). Conclusion. The mechanism by which the nourishing “Yin” removing “Fire” Chinese herbal mixture delays puberty onset may be associated with the inhibition of the hypothalamic mTOR signaling.