A MUTANT NUCLEAR-PROTEIN WITH SIMILARITY TO RNA-BINDING PROTEINS INTERFERES WITH NUCLEAR IMPORT IN YEAST
A MUTANT NUCLEAR-PROTEIN WITH SIMILARITY TO RNA-BINDING PROTEINS INTERFERES WITH NUCLEAR IMPORT IN YEAST
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DOI：
10.1091/mbc.3.8.875
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发表时间：
1992-08-01
影响因子：
3.3
通讯作者：
SILVER, P
中科院分区：
文献类型：
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作者：
BOSSIE, MA;DEHORATIUS, C;SILVER, P
我们分离出了酿酒酵母的突变体，其核蛋白定位有缺陷。含有融合到线粒体 F1-β-ATP 酶 N 末端的 SV40 大 T 抗原的核定位序列的嵌合蛋白定位于细胞核。 Npl（核蛋白定位）突变体因其在甘油上生长的能力而被分离出来，因为它们不再专门将 SV40-F1-β-ATP 酶靶向细胞核。所有核仁蛋白和组蛋白定位缺陷的突变体都对 36 摄氏度的生长温度敏感。分离出 NPL3 的七个等位基因和几个其他基因的单个等位基因。对 NPL3 突变体进行了详细研究。 NPL3 编码具有 RNA 识别基序的核蛋白，并且与参与 RNA 代谢的蛋白质家族相似。我们的遗传分析表明 NPL3 对于正常细胞生长至关重要；缺乏 NPL3 的细胞对生长对温度敏感，但在核蛋白定位方面不表现出缺陷。总而言之，这些结果表明通过该程序分离的Npl3蛋白的突变形式以一般方式干扰核蛋白摄取。
We have isolated mutants of the yeast Saccharomyces cerevisiae that are defective in localization of nuclear proteins. Chimeric proteins containing the nuclear localization sequence from SV40 large T-antigen fused to the N-terminus of the mitochondrial F1-beta-ATPase are localized to the nucleus. Npl (nuclear protein localization) mutants were isolated by their ability to grow on glycerol as a consequence of no longer exclusively targeting SV40-F1-beta-ATPase to the nucleus. All mutants with defects in localization of nucleolar proteins and histones are temperature sensitive for growth at 36-degrees-C. Seven alleles of NPL3 and single alleles of several additional genes were isolated. NPL3 mutants were studied in detail. NPL3 encodes a nuclear protein with an RNA recognition motif and similarities to a family of proteins involved in RNA metabolism. Our genetic analysis indicates that NPL3 is essential for normal cell growth; cells lacking NPL3 are temperature sensitive for growth but do not exhibit a defect in localization of nuclear proteins. Taken together, these results indicate that the mutant forms of Npl3 protein isolated by this procedure are interfering with nuclear protein uptake in a general manner.