Localization by mutational analysis of transcription factor binding sequences in the U3 region of Rous sarcoma virus LTR.
Localization by mutational analysis of transcription factor binding sequences in the U3 region of Rous sarcoma virus LTR.
复制标题
通过劳斯肉瘤病毒 LTR U3 区域转录因子结合序列的突变分析进行定位。
DOI：
10.1016/0042-6822(90)90018-m
复制
发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
Guntaka,RV
中科院分区：
文献类型：
--
作者：
Kenny,S;Guntaka,RV
在劳斯肉瘤病毒LTR的U3区的转录因子结合序列已确定的凝胶阻滞试验使用突变的合成寡核苷酸。结果表明，E2 BP因子特异性结合于TGCAATAC和TGCAACAT序列，其分别位于核苷酸-222至-215和-203至-196之间。与小鼠3 T3和大鼠2 tk−细胞相比，该因子在禽类QT 6细胞中的水平升高。E2 BP结合与大鼠肝C/EBP识别的序列相似或相同的序列。然而，这两种蛋白质是不同的，如通过三个标准判断的：（i）E2 BP复合物比E2-C/EBP复合物迁移稍快;（ii）针对C/EBP的抗体既不抑制E2 BP的结合，也不形成比单独与因子形成的复合物迁移更慢的超复合物;和（iii）E2 BP是热不稳定的，而C/EBP是热稳定的。另一种因子E3 BP，其结合U3区-169至-158的序列，也主要在QT 6细胞中检测到，但在小鼠或大鼠细胞中未检测到。这些结果表明，不同的细胞特异性因子与RSV LTR U3区不同的顺式作用调控序列相互作用。
The transcription factor binding sequences in the U3 region of Rous Sarcoma virus LTR have been determined by gel retardation assays using mutant synthetic oligonucleotides. The results indicate that the factor, E2BP, specifically binds to sequences TGCAATAC and TGCAACAT, which are localized between nucleotides -222 to -215 and -203 to -196, respectively. This factor is present at elevated levels in avian QT6 cells compared to mouse 3T3 and rat 2 tk−cells. E2BP binds to a sequence that is similar or identical to the sequence recognized by rat liver C/EBP. However, the two proteins are different as judged by three criteria: (i) the E2BP complex migrates slightly faster than the E2-C/EBP complex; (ii) antibodies against C/EBP neither inhibit binding of E2BP nor form a supercomplex which migrates slower than the complex formed with the factor alone; and (iii) E2BP is heat labile whereas C/EBP is heat stable. Another factor, E3BP, which binds to a sequence from -169 to -158, in the U3 region is also detected mainly in QT6 cells but not in mouse or rat cells. These results suggest that different cell-specific factors interact with differentcis-acting regulatory sequences in the U3 region of RSV LTR.