Effect of Tissue Specificity on the Performance of Extracellular Matrix in Improving Endothelialization of Cardiovascular Implants
Effect of Tissue Specificity on the Performance of Extracellular Matrix in Improving Endothelialization of Cardiovascular Implants
复制标题
组织特异性对细胞外基质改善心血管植入物内皮化性能的影响
DOI：
10.1089/ten.tea.2011.0372
复制
发表时间：
2013-01-01
影响因子：
4.1
通讯作者：
Lei, Yuechang
中科院分区：
文献类型：
--
作者：
Tu, Qiufen;Yang, Zhilu;Lei, Yuechang
在我们以前的研究中，由培养的内皮细胞（ECs）原位沉积的天然细胞外基质（ECM）已被证明可以有效地加速钛（Ti）心血管植入物（CVI）的内皮化。在这项研究中，ECM沉积的平滑肌细胞（SMCs）的比较，研究ECM的组织特异性的能力，加速内皮化的CVIs的影响。结果表明，由EC和SMC沉积的ECM（分别为EC-ECM和SMC-ECM）在成分和纤维形态上有很大差异。用两种天然ECM对Ti CVIs进行表面改性可同时有效改善其体外血液相容性和细胞相容性。然而，在犬模型中ECM修饰的Ti CVI的内皮化显示出ECM的高组织特异性。虽然由SMC沉积的ECM（SMC-ECM）在体外诱导较少的血小板粘附并维持EC的更好的生长和活力，但其在体内加速Ti CVIs的内皮化的性能极差。相反，由EC沉积的ECM（EC-ECM）导致体内完全内皮形成。
Natural extracellular matrix (ECM) deposited in situ by cultured endothelial cells (ECs) has been proven effective in accelerating endothelialization of titanium (Ti) cardiovascular implants (CVIs) in our previous studies. In this study, the ECM deposited by smooth muscle cells (SMCs) was used in comparison to investigate the effects of tissue specificity of the ECM on the ability to accelerate endothelialization of CVIs. The results demonstrated that the ECM deposited by ECs and SMCs (EC-ECM, SMC-ECM, respectively) differed considerably in components and fibril morphology. Surface modification of Ti CVIs with both types of natural ECM was effective in improving their in vitro hemocompatibility and cytocompatibility simultaneously. However, the endothelialization of ECM-modified Ti CVIs in a canine model demonstrated a high tissue specificity of the ECM. Although the ECM deposited by SMCs (SMC-ECM) induced fewer platelet adhesion and sustained better growth and viability of ECs in vitro, its performance in accelerating in vivo endothelialization of Ti CVIs was extremely poor. In contrast, the ECM deposited by ECs (EC-ECM) led to complete endothelium formation in vivo.