Matrigel coated polydimethylsiloxane based microfluidic devices for studying metastatic and non-metastatic cancer cell invasion and migration
Matrigel coated polydimethylsiloxane based microfluidic devices for studying metastatic and non-metastatic cancer cell invasion and migration
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DOI：
10.1007/s10544-007-9071-5
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发表时间：
2007-08-01
影响因子：
2.8
通讯作者：
Swaminathan, S.
中科院分区：
文献类型：
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作者：
Chaw, K. C.;Manimaran, M.;Swaminathan, S.
三维(3-D)细胞外基质(ECM)允许细胞和基质之间复杂的生化和生物物理相互作用。与二维系统不同，三维模型能更好地表示活组织中的微环境和局部环境，便于对细胞迁移的生理学研究。在这里，我们报告了一种基于聚二甲基硅氧烷(PDMS)的微流控装置，用于用实时光学显微镜监测三维细胞在ECM涂层微隙中的迁移。我们跟踪了侵袭性的MDA-MB-231(乳腺癌)细胞的迁移，并绘制了它们的迁移路径。它使我们能够量化迁移细胞的百分比以及单个细胞的迁移信息。这种广谱的数据采集对于阐明不同类型细胞的迁移能力和了解癌症转移的基本机制至关重要。
Three-dimensional (3-D) extracellular matrices (ECM) allow complex biochemical and biophysical interactions between cells and matrices. Unlike 2-D systems, 3-D models provide a better representation of the micro and local environments in living tissues for facilitating the physiological study of cell migration. Here, we report a microfluidic device based on olydimethylsiloxane (PDMS) for monitoring 3-D cell migration across ECM-coated microgaps with real-time light microscopy. We tracked the migration of the invasive MDA-MB-231 (mammary carcinoma) cells and mapped out their migration paths. It enabled us to quantify the percentage of migrated cells as well as migration information of individual cells. This wide spectrum of data acquisition is vital for elucidating the migration capabilities of different type of cells and to understand the basic mechanism involved in cancer metastasis.