Immunosuppressive activity of serum from liver-grafted rats: in vitro specific inhibition of mixed lymphocyte reactivity by antibodies against class II RT1 alloantigens.
Immunosuppressive activity of serum from liver-grafted rats: in vitro specific inhibition of mixed lymphocyte reactivity by antibodies against class II RT1 alloantigens.
复制标题
肝移植大鼠血清的免疫抑制活性：针对 II 类 RT1 同种异体抗原的抗体对混合淋巴细胞反应性的体外特异性抑制。
作者：
M. Tsurufuji;K. Ishiguro;T. Shinomiya;T. Uchida;N. Kamada
体外检测了DA肝脏原位移植后PVG大鼠血清的免疫抑制活性。这种组合中的肝移植物从未被排斥，但在受体中诱导特异性移植耐受的状态。来自这种耐受动物的血清能够抑制正常PVG淋巴结细胞在混合淋巴细胞反应（MLR）中对DA刺激物的增殖;抑制对供体（DA）抗原具有特异性。MLR期间白细胞介素-2（IL-2）的产生也减少。在MLR中发育的抗DA细胞毒性T细胞的产生不受影响，但此类细胞的总产量降低。有证据表明，部分抑制血清活性是由于IgG抗体对II类RT 1a同种异体抗原。因此，纯化的IgG级分保留了大部分的抑制活性，其可以通过抗IgG吸收剂去除。在不同大鼠品系中的MLR抑制研究表明抑制性IgG的抗II类特异性。DA-肝移植PVG大鼠的淋巴结细胞在体外对DA刺激物反应正常，并且这种MLR也被抑制性IgG阻断。我们的研究结果表明，抗II类同种抗体可能在免疫抑制和肝移植后的长期存活中发挥作用。
The immunosuppressive activity of serum from PVG rats following orthotopic transplantation of DA liver has been examined in vitro. Liver grafts in this combination are never rejected, but induce a state of specific transplantation tolerance in the recipient. Serum from such tolerant animals was able to inhibit proliferation of normal PVG lymph node cells in response to DA stimulators in the mixed lymphocyte reaction (MLR); inhibition was specific for donor (DA) antigens. Interleukin-2 (IL-2) production during the MLR was also reduced. The production of anti-DA cytotoxic T cells developing in the MLR was not affected, but the total yield of such cells was reduced. Evidence was obtained that part of the inhibitory serum activity was due to IgG antibody against class II RT1a alloantigens. Thus, a purified IgG fraction retained much of the inhibitory activity which could be removed by an anti-IgG absorbent. Studies of MLR inhibition in different rat strains indicated the anti-class II specificity of the inhibitory IgG. Lymph node cells from DA-liver-grafted PVG rats responded normally against DA stimulators in vitro, and this MLR was also blocked by the inhibitory IgG. Our results suggest that anti-class II allo-antibody may play a role in immunosuppression and long-term graft survival following liver transplantation in this combination.