SERTOLI CELLS CONTAIN A MITOGENIC POLYPEPTIDE
SERTOLI CELLS CONTAIN A MITOGENIC POLYPEPTIDE
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DOI：
10.1073/pnas.77.8.4774
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
KLAGSBRUN, M
中科院分区：
文献类型：
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作者：
FEIG, LA;BELLVE, AR;KLAGSBRUN, M
小鼠睾丸的生精上皮含有一种有效的有丝分裂原，可诱导融合静止小鼠成纤维细胞BALB/c 3 T3细胞培养物中的DNA合成和细胞分裂。当将成年小鼠生精小管的匀浆加入3 T3细胞中48小时时，产生DNA合成的剂量依赖性增加，在400 μ g蛋白质/ml时具有半最大活性。最大刺激导致[甲基-3H]胸苷掺入DNA增加70至100倍，标记> 99%的3 T3细胞核，细胞数量加倍。从新生小鼠分离的曲细精管中的促有丝分裂活性水平是成年小鼠曲细精管中的促有丝分裂活性水平的5倍。青春期前的支持细胞比从生精上皮纯化的任何其他细胞类型含有更高水平的促有丝分裂活性。青春期前Sertoli细胞的匀浆在20 μ g蛋白质/ml下诱导半最大DNA合成。促有丝分裂因子对蛋白酶和100 ℃加热敏感。但不与二硫苏糖醇反应。凝胶过滤层析显示该因子是一种分子量为15，500的多肽。这些观察结果为哺乳动物睾丸中存在生长因子提供了证据。
The seminiferous epithelium of the mouse testis contains a potent mitogen that induces DNA synthesis and cell division in cultures of confluent, quiescent mouse fibroblast BALB/c 3T3 cells. Homogenates of adult mouse seminiferous tubules, when added to 3T3 cells for 48 h, produce a dose-dependent increase in DNA synthesis with half-maximal activity at 400 .mu.g of protein/ml. Maximal stimulation causes a 70- to 100-fold increase in [methyl-3H]thymidine incorporation into DNA, a labeling of > 99% of 3T3 cell nuclei, and a doubling in cell number. The level of mitogenic activity in seminiferous tubules isolated from newborn mice is 5 times greater than that in tubules of adult mice. Prepuberal Sertoli cells contain substantially greater levels of mitogenic activity than any other cell type purified from the seminiferous epithelium. A homogenate of prepuberal Sertoli cells induces half-maximal DNA synthesis at 20 .mu.g of protein/ml. The mitogenic factor is sensitive to proteases and to heating at 100.degree. C for 2 min but not to dithiothreitol. Gel-filtration chromatography reveals that the factor is a polypeptide with a MW of 15,500. These observations provide evidence for the presence of a growth factor in the mammalian testis.