Vaccine production in Neurospora crassa
Vaccine production in Neurospora crassa
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DOI：
10.1016/j.biologicals.2009.02.006
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发表时间：
2009-06-01
期刊：
影响因子：
1.7
通讯作者：
Stuart, W. Dorsey
中科院分区：
文献类型：
--
作者：
Allgaier, Silke;Taylor, Rebecca D.;Stuart, W. Dorsey
我们选择使用丝状真菌粗神经孢子菌来生产亚单位疫苗。本文描述了流行性感冒血凝素和神经氨酸酶抗原的生产和纯化。Neugenesis使用的N. crassa系统在生产重组蛋白方面比其他系统有许多优点。与哺乳动物细胞培养不同的是，N. crassa可以以快速和经济的方式生长，在简单、明确的培养基中产生大量重组蛋白。因此，与传统的细胞培养或基于鸡蛋的系统相比，疫苗的生产速度更快，成本更低。这在调整季节性疫苗供应和应对新的大流行方面具有重要应用。(C) 2009年由Elsevier Ltd代表国际生物制品协会出版。
We have chosen to use the filamentous fungus Neurospora crassa to produce subunit vaccines. Here we describe the production and purification of Influenza hemagglutinin and neuraminidase antigens in N. crassa. The N. crassa system used by Neugenesis offers many advantages over other systems for production of recombinant protein. In contrast to mammalian cell culture, N. crassa can be grown in a rapid and economic manner, generating large amounts of recombinant protein in simple, defined medium. Vaccines, therefore, can be produced more rapidly and at lower cost than conventional cell culture or egg-based systems. This has important applications to tailoring the seasonal vaccine supply and responding to new pandemics. (C) 2009 Published by Elsevier Ltd on behalf of The International Association for Biologicals.