The glutamate decarboxylase-, leucine enkephalin-, methionine enkephalin- and substance P-immunoreactive neurons in the neostriatum of the rat and cat: evidence for partial population overlap.
The glutamate decarboxylase-, leucine enkephalin-, methionine enkephalin- and substance P-immunoreactive neurons in the neostriatum of the rat and cat: evidence for partial population overlap.
复制标题
大鼠和猫新纹状体中谷氨酸脱羧酶、亮氨酸脑啡肽、蛋氨酸脑啡肽和 P 物质免疫反应性神经元：部分群体重叠的证据。
DOI：
10.1016/0306-4522(86)90076-x
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发表时间：
1986
期刊：
影响因子：
3.3
通讯作者：
Kitai,ST
中科院分区：
文献类型：
--
作者：
Penny,GR;Afsharpour,S;Kitai,ST
我们研究了大鼠和猫新纹状体中谷氨酸脱羧酶、亮氨酸脑啡肽、甲硫氨酸脑啡肽和P物质免疫阳性神经元的数量。谷氨酸脱羧酶阳性神经元占大鼠标本神经元的47%，占猫神经元总数的39%~49%。[Leu]脑啡肽免疫阳性神经元占大鼠新纹状体神经元的44-49%，猫的占38-47%；[Met]脑啡肽免疫阳性的神经元占大鼠神经元的36-41%，猫的神经元的43%-49%。P物质免疫反应阳性神经元占大鼠神经元的30-38%，猫的32-39%，但大多数P物质神经元(特别是染色最深的神经元)聚集在新纹状体的斑块间，在斑块内，P物质神经元占大鼠神经元的59%，猫的55%，而在基质中，P物质神经元仅占大鼠神经元的32%，猫的25%。结果表明，12%的神经元同时标记谷氨酸脱羧酶和亮氨酸脑啡肽，12%的神经元同时标记谷氨酸脱羧酶和P物质，11%~12%的神经元同时标记P物质和P物质，17%的神经元同时标记P物质和P物质。这些结果为中等大小的新纹状体神经元内存在化学异质性提供了证据，并首次证明了P物质和P物质在中枢神经系统的单个神经元内共存。
We have examined the populations of neurons in the neostriatum of both rat and cat that are immunoreactive for glutamate decarboxylase, [Leu]enkephalin, [Met]enkephalin and substance P. Neurons that were immunoreactive for glutamate decarboxylase made up 47% of the neurons in our samples from the rat and ranged from 39 to 49% of the neurons in the cat. Those immunoreactive for [Leu]enkephalin made up 44–49% of the neurons in rat neostriatum, and 38–47% in the cat, and those immunoreactive for [Met]enkephalin made up 36–41% of the neurons in rat and 43–49% of the neurons in the cat. Substance P-immunoreactive neurons made up 30–38% of neurons in rat and 32–39% in cat Most substance P neurons (particularly the most darkly staining ones) were, however, clustered such that they were most numerous in the patch compartment of neostriatum; within the patches the substance P neurons comprised 59% of neurons in the rat and 55% in cat, but in the matrix substance P neurons comprised only 32% of neurons in the rat and 25% in the cat. Samples taken from sections processed for two-color double labeling immunocytochemistry revealed that 12% of neurons label for both glutamate decarboxylase and [Leu]enkephalin, 12% for both glutamate decarboxylase and [Met]enkephalin, 11–12% for both glutamate decarboxylase and substance P, and 17% for both [Met]enkephalin and substance P.These results provide evidence for chemical heterogeneity within the medium-sized neostriatal neurons, and provide the first evidence for coexistence of glutamate decarboxylase and substance P within a single neuron, and the first evidence for the coexistence for substance P and [Met]enkephalin within single neurons of the central nervous system.