Correlation of Antithrombogenicity and Heat Treatment for Layer-by-Layer Self-Assembled Polyelectrolyte Films
Correlation of Antithrombogenicity and Heat Treatment for Layer-by-Layer Self-Assembled Polyelectrolyte Films
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层层自组装聚电解质薄膜的抗血栓性与热处理的相关性
DOI：
10.1021/la200340v
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发表时间：
2011
期刊：
影响因子：
3.9
通讯作者：
Seimei Shiratori
中科院分区：
文献类型：
--
作者：
Motomi Matsuda;Seimei Shiratori
在湿法工艺中制备具有高耐久性的抗血栓形成膜。抗血栓性是通过由聚乙烯醇-聚丙烯酸（PVA-PAA）共混物制备的多层薄膜实现的，该共混物与聚烯丙胺盐酸盐（PAH）交替沉积。膜的耐久性，通过耐磨性和耐水性评估，通过形成交联通过酰胺键引起的膜的热处理。该膜被发现是耐蛋白质吸附，如从水溶液中吸附的纤维蛋白原的量所测量。在离体实验中，通过涂覆有明胶并插入猪血管中的不锈钢网抑制血栓形成的能力来评估抗血栓形成功效。涂有明胶的补片在15分钟内没有减少血流，而未涂敷的补片由于血栓形成导致血管阻塞，血流在6分钟内停止。
Antithrombogenic films with high durability were fabricated in a wet process. Antithrombogenicity was achieved with polyelectrolyte multilayer thin film prepared from poly(vinyl alcohol)−poly(acrylic acid) (PVA−PAA) blends, deposited in alternate layers with poly(allylamine hydrochloride) (PAH). Film durability, assessed by abrasion resistance and water resistance, was enhanced by forming cross-links via amide bonds induced by heat treatment of the film. The film was found to be resistant to protein adsorption, as measured by the amount of fibrinogen adsorbed from an aqueous solution. The antithrombogenic efficacy was assessed in ex vivo experiments by the ability of stainless steel mesh, coated with the polyelectrolyte and inserted into a pig blood vessel, to inhibit thrombus formation. Mesh coated with the polyelectrolyte did not reduce blood flow over a period of 15 min, whereas with uncoated mesh blood flow stopped within 6 min because of blood vessel blockage by thrombus formation.