Quantitative Biomechanics of Healthy and Diseased Human Red Blood Cells using Dielectrophoresis in a Microfluidic System.
Quantitative Biomechanics of Healthy and Diseased Human Red Blood Cells using Dielectrophoresis in a Microfluidic System.
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DOI：
10.1016/j.eml.2014.11.006
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发表时间：
2014-12
影响因子：
4.7
通讯作者：
Suresh, Subra
中科院分区：
文献类型：
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作者：
Du, E;Dao, Ming;Suresh, Subra
我们提出了一种实验方法，通过在微流控装置中引入控制变形来定量表征大量生物细胞的力学特性。我们通过测定健康人红细胞（红细胞）和体外感染恶性疟原虫的红细胞的受力与变形特性来证明这种技术的能力。这些实验清楚地区分了未感染和健康的红细胞与感染的红细胞，从这些测试中提取的机械特征与其他独立方法的数据一致。因此，本文开发的方法提供了一种潜在的有用工具，可以快速有效地表征大量细胞的分离生物力学反应，用于探测细胞的病理状态，疾病诊断和药物疗效分析。
We present an experimental method to quantitatively characterize the mechanical properties of a large number of biological cells by introducing controlled deformation through dielectrophoresis in a microfluidic device. We demonstrate the capability of this technique by determining the force versus deformation characteristics of healthy human red blood cells (RBCs) and RBCs infected in vitro with Plasmodium falciparum malaria parasites. These experiments clearly distinguish uninfected and healthy RBCs from infected ones, and the mechanical signatures extracted from these tests are in agreement with data from other independent methods. The method developed here thus provides a potentially helpful tool to characterize quickly and effectively the isolated biomechanical response of cells in a large population, for probing the pathological states of cells, disease diagnostics, and drug efficacy assays.