Ribosomic DNA intergenic spacer 1 region is useful when identifying Candida parapsilosis spp. complex based on high-resolution melting analysis
Ribosomic DNA intergenic spacer 1 region is useful when identifying Candida parapsilosis spp. complex based on high-resolution melting analysis
复制标题
核糖体 DNA 基因间间隔区 1 区域在鉴定近平滑念珠菌属时非常有用。
DOI：
10.1093/mmy/myu009
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发表时间：
2014
期刊：
影响因子：
2.9
通讯作者：
Gago S
中科院分区：
文献类型：
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作者：
Gago S
近丝念珠菌及其近缘种C.正硅酸盐和C. metapsilosishas近年来发生了变化，证明需要在物种水平上识别这种复合体。在这项研究中，我们调查了核糖体DNA（rDNA）的基因间间隔区1（IGS1），以评估该基因区域作为系统发育分子标记的实用性和基于该区域的高分辨率熔解（HRM）策略用于鉴定C.近沟藻属复杂.我们对33 C的rDNA的IGS1和ITS区进行了测序。感觉近平滑虽然这两个区域是有用的，在确定物种，比较序列分析表明，在IGS1区域的多样性高于ITS序列。我们还开发了一个人力资源管理分析，可靠地识别C。近沟藻属基于IGS1区域中70 bp的扩增的复合物。所有分离株均正确鉴定，置信区间> 98%。我们的研究结果表明，HRM分析的基础上IGS1区域是一个强大的工具，用于预测C。parasilosisfrom cryptic神秘species种.
The epidemiology ofCandida parapsilosisand the closely related speciesC. orthopsilosisandC. metapsilosishas changed in recent years, justify the need to identify this complex at the species level. In this study we investigate the intergenic spacer 1 (IGS1) of the ribosomal DNA (rDNA) to evaluate the utility of this gene region as a phylogenetic molecular marker and the suitability of a high-resolution melting (HRM) strategy based on this region for identification of members of theC. parapsilosisspp. complex. We sequenced the IGS1 and the internal transcribed spacer (ITS) regions of the rDNA from 33C. parapsilosis sensu latostrains. Although both regions are useful in identifying species, comparative sequence analysis showed that the diversity in the IGS1 region was higher than in the ITS sequences. We also developed an HRM analysis that reliably identifiesC. parapsilosisspp. complex based on the amplification of 70 bp in the IGS1 region. All isolates were correctly identified with a confidence interval >98%. Our results demonstrate that HRM analysis based on the IGS1 region is a powerful tool for distinguishingC. parapsilosisfrom cryptic species.