Effects of Angelicae dahuricae Radix on 2, 4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in mice model.
Effects of Angelicae dahuricae Radix on 2, 4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in mice model.
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DOI：
10.1186/s12906-017-1584-8
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发表时间：
2017-02-07
影响因子：
--
通讯作者：
Ko SG
中科院分区：
文献类型：
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作者：
Ku JM;Hong SH;Kim HI;Seo HS;Shin YC;Ko SG
特应性皮炎(AD)是一种炎症性、慢性复发和强烈瘙痒的皮肤病，影响全球人口的10%-30%。据韩国医学报道，白藜芦醇(ADR)具有抗炎作用。在本研究中，我们在动物模型上研究了ADR是否能抑制AD的进展。用2，4-二硝基氯苯(DNCB)诱导AD。用小鼠灌胃ADR，研究ADR对AD的影响。进行组织学分析、免疫组织化学、血液分析、RT-PCR和ELISA法检测。ADR可显著抑制BALB/c小鼠的AD样症状：ADR可使小鼠皮肤厚度和脾重量减少。ADR可减少肥大细胞、炎性细胞和CD4+细胞在小鼠皮肤中的渗透。ADR可降低小鼠血中WBC的数量。ADR可降低小鼠血清中IgE、IL-6、IL-10、IL-12水平。阿霉素可下调小鼠皮肤组织中IL-4、IL-6和肿瘤坏死因子-α的基因表达。我们目前的研究清楚地表明，ADR可以抑制DNCB诱导的BALB/c小鼠AD的进展。提示ADR可能是治疗AD的有效药物。
Atopic dermatitis (AD) is an inflammatory, chronically relapsing, and intensively pruritic skin disease that affect 10–30% of the global population. Angelicae dahuricae Radix (ADR) has been reported to be anti-inflammatory in Korean Medicine. In the present study, we investigated whether ADR suppresses the progression of AD in animal model. AD was induced by 2, 4-Dinitrochlorobenzene (DNCB). ADR was orally administered to mice to study the effect of ADR on AD. Histological Analysis, immunohistochemistry, blood analysis, RT-PCR, and ELISA assay were performed. ADR significantly suppressed AD-like symptoms in BALB/c mice: ADR decreased skin thickness and spleen weight of mice. ADR reduced infiltration of mast cells, inflammatory cells and CD4+ cells into mouse skin. ADR lowered the number of WBCs in the blood of mice. ADR reduced the levels of IgE, IL-6, IL-10 and IL-12 in mice serum. ADR down-regulated mRNA expression of IL-4, IL-6 and TNF-α in mouse skin tissue. Our present study clearly indicates that ADR suppresses the progression of AD induced by DNCB in BALB/c mice. This suggests that ADR might be a useful drug for the treatment of AD.