Design, preparation and measurement of protein/CNTs hybrids: A concise review
Design, preparation and measurement of protein/CNTs hybrids: A concise review
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DOI：
10.1016/j.jmst.2020.01.008
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发表时间：
2020-06
影响因子：
10.9
通讯作者：
Xing Zhou;Jian Su;Chenxi Wang;Changqing Fang;Xinyu He;Wanqing Lei;Chaoqun Zhang;Zhigang Huang
中科院分区：
文献类型：
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作者：
Xing Zhou;Jian Su;Chenxi Wang;Changqing Fang;Xinyu He;Wanqing Lei;Chaoqun Zhang;Zhigang Huang
随着纳米生物技术的发展，碳纳米管（CNTs）与蛋白质的杂化体系在纳米技术、医学、智能材料、轻工业、生物学等领域具有巨大的应用潜力，受到越来越多的关注。本文从实验角度介绍了碳纳米管/蛋白质的主要制备工艺、影响因素、相互作用的测量以及潜在的应用。同时，对合适的力检测方法进行了全面的阐述，以完成定量测量。原子力显微镜（AFM）和表面力仪（SFA）的实验进行了详细的描述，以证实在测量相互作用力的强大功能。此外，还讨论了不同蛋白质结构（氨基酸残基、α-组氨酸、多肽链和组装亚基）对碳纳米管与蛋白质相互作用的影响，不同氨基酸残基对相互作用的影响很大。由于对蛋白质与碳纳米管表面相互作用的结构、功能和空间取向的了解相对较少，我们认为关键问题是如何制备具有独特结构的碳纳米管和蛋白质样品（如蛋白质的二级和三维结构的变化或单个碳纳米管）来研究相互作用力，而不是设计、制备和检测方法。
With the development of nanobiotechnology, the carbon nanotubes (CNTs) and protein hybrids system has attracted an increasing attention for great potential application in nanotechnology, medicine, smart materials, light industry, and biology. In this review, the main preparation processes, impact factors, measurement of interactions and potential applications of CNTs/protein are presented from the aspect of experiments. Meanwhile, the proper forces detection methods are illustrated comprehensively to complete the quantitative measurement. Atomic force microscope (AFM) and surface force apparatus (SFA) experiments are described in detail to confirm the powerful function in measuring the interaction forces. In addition, the impact of different protein structures (amino acid residues, α Helix, polypeptide chain, and assembled subunits) on interactions between CNTs and protein is presented and different amino acid residues may intervene largely on interactions. Owing to the relatively little knowledge about the structure, function, and spatial orientation of proteins interaction with CNTs surface, we assume that the key problem is how to prepare CNTs and protein specimen with unique structure (such as the variation of secondary and tridimensional structure of protein or the single CNTs) to investigate the interaction forces instead of the designed, preparation, and detection methods.