A Three-State Mathematical Model of Hyperthermic Cell Death
A Three-State Mathematical Model of Hyperthermic Cell Death
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DOI：
10.1007/s10439-010-0177-1
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发表时间：
2011-01-01
影响因子：
3.8
通讯作者：
Payne, Stephen J.
中科院分区：
文献类型：
--
作者：
O'Neill, David P.;Peng, Tingying;Payne, Stephen J.
用于组织消融的热处理依赖于加热细胞超过阈值，超过该阈值，细胞被认为被破坏、变性或杀死。在这篇文章中，提出了一种新的三态细胞死亡模型，其中存在一个脆弱的状态之间的生存和死亡状态中使用的一些现有的细胞死亡模型。建议的速率系数包括温度依赖性和模型拟合的肝细胞和肺成纤维细胞的加热共培养物的实验数据具有非常小的RMS误差。所利用的实验数据包括加热后24和48小时内细胞活力的进一步降低，这些数据用于扩展三态模型以解释缓慢的细胞死亡。对于实验数据中使用的两种细胞系，快速细胞死亡的三个参数似乎随肺成纤维细胞的%含量线性增加，而数据的稀疏性并未表明缓慢细胞死亡参数的任何共培养组成依赖性。提出了一个关键的加热后细胞活力阈值，超过该阈值，细胞将死亡;这些结果具有实际意义，具有更准确预测细胞死亡的潜力。
Thermal treatments for tissue ablation rely upon the heating of cells past a threshold beyond which the cells are considered destroyed, denatured, or killed. In this article, a novel three-state model for cell death is proposed where there exists a vulnerable state positioned between the alive and dead states used in a number of existing cell death models. Proposed rate coefficients include temperature dependence and the model is fitted to experimental data of heated co-cultures of hepatocytes and lung fibroblasts with very small RMS error. The experimental data utilized include further reductions in cell viabilities over 24 and 48 h post-heating and these data are used to extend the three-state model to account for slow cell death. For the two cell lines employed in the experimental data, the three parameters for fast cell death appear to be linearly increasing with % content of lung fibroblast, while the sparse nature of the data did not indicate any co-culture make-up dependence for the parameters for slow cell death. A critical post-heating cell viability threshold is proposed beyond which cells progress to death; and these results are of practical importance with potential for more accurate prediction of cell death.