An aquaporin protein is associated with drought stress tolerance
An aquaporin protein is associated with drought stress tolerance
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水通道蛋白与干旱胁迫耐受性相关
DOI：
10.1016/j.bbrc.2015.02.052
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发表时间：
2015-04-03
影响因子：
3.1
通讯作者：
Wang, Xuemin
中科院分区：
文献类型：
--
作者：
Li, Jun;Ban, Liping;Wang, Xuemin
被称为水通道蛋白（AQPs）的水通道蛋白调节水和其他小分子在植物液泡膜和质膜上的移动；它们与植物对生物和非生物胁迫的耐受性有关。在本研究中，从一种高价值的豆科牧草——东方山羊豆（Galega orientalis）中克隆出一个PIP型水通道蛋白基因，命名为GoPIP1。GoPIP1基因包含一个870bp的开放阅读框，编码一个含289个氨基酸的蛋白质，属于PIP亚家族的PIP1亚组。GoPIP1在东方山羊豆根中的转录水平高于叶和茎。当用200 mM NaCl或20%聚乙二醇（PEG）6000处理时，GoPIP1转录本的水平显著增加。GoPIP1在洋葱表皮细胞中的瞬时表达表明GoPIP1蛋白定位于质膜。GoPIP1的过表达增加了转基因拟南芥植株的莲座叶/根的比例，并增加了对干旱的敏感性。然而，在盐胁迫条件下，GoPIP1在拟南芥中的过表达没有显著影响。目前的数据提供了一种基因资源，有助于加深我们对水通道蛋白及其在植物抗逆性中的应用的理解。（C）2015作者。由爱思唯尔公司出版。
Water channel proteins known as aquaporins (AQPs) regulate the movement of water and other small molecules across plant vacuolar and plasma membranes; they are associated with plant tolerance of biotic and abiotic stresses. In this study, a PIP type AQPs gene, designated as GoPIP1, was cloned from Galega orientalis, a high value leguminous forage crop. The GoPIP1 gene consists of an 870 bp open reading frame encoding a protein of 289 amino acids, and belongs to the PIP1 subgroup of the PIP subfamily. The transcript level of GoPIP1 was higher in the root of G. orientalis than in the leaf and stem. The level of GoPIP1 transcript increased significantly when treated with 200 mM NaCl or 20% polyethylene glycol (PEG) 6000. Transient expression of GoPIP1 in onion epidermal cells revealed that the GoPIP1 protein was localized to the plasma membrane. Over-expression of GoPIP1 increased the rosette/root ratio and increased sensitivity to drought in transgenic Arabidopsis plants. However, GoPIP1 overexpression in Arabidopsis had no significant effect under saline condition. The present data provides a gene resource that contributes to furthering our understanding of water channel protein and their application in plant stress tolerance. (C) 2015 The Authors. Published by Elsevier Inc.