Alterations in novel candidate tumor suppressor genes, ING1 and ING2 in human lung cancer.
Alterations in novel candidate tumor suppressor genes, ING1 and ING2 in human lung cancer.
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DOI：
10.3892/or.15.3.545
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发表时间：
2006-03
期刊：
影响因子：
4.2
通讯作者：
T. Okano;A. Gemma;Y. Hosoya;Y. Hosomi;M. Nara;Y. Kokubo;A. Yoshimura;M. Shibuya;M. Nagashima;C. Harris;S. Kudoh
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文献类型：
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作者：
T. Okano;A. Gemma;Y. Hosoya;Y. Hosomi;M. Nara;Y. Kokubo;A. Yoshimura;M. Shibuya;M. Nagashima;C. Harris;S. Kudoh
ING1基因参与细胞周期、衰老和凋亡的调控，是一种新的候选肿瘤抑制基因。ING家族的另一个基因ING2被鉴定并克隆。ING1和ING2的功能很大程度上取决于p53的活性。为了确定这些基因的改变是否在肺癌的癌变和肿瘤进展中起作用，我们使用聚合酶链反应-单链构象多态性（PCR-SSCP）和直接测序技术筛选了30个人类肺癌细胞系和31个原发性肺癌肿瘤，以检测这些基因的突变。我们的发现未能发现这些基因中的任何突变。我们还使用实时定量逆转录聚合酶链反应（RT-PCR）检测了ING1和ING2在有或缺乏p53突变的肺癌细胞系和对照支气管上皮细胞系中的表达。在p53突变的7株肺癌细胞系中，ING1的表达均上调，而在p53突变的7株肺癌细胞系中，有6株的ING2表达下调。这些结果提示ING1和ING2基因在肺癌的发生和发展中具有不同的作用，ING2基因可能是p53的独立抑癌候选基因。
The ING1 gene is involved in the regulation of the cell cycle, senescence, and apoptosis and is a novel candidate tumor suppressor gene. ING2, another gene in the ING family, was identified and cloned. The functions of ING1 and ING2 largely depend on the activity of p53. To determine whether an alteration in these genes plays a role in carcinogenesis and tumor progression in lung cancer, we screened 30 human lung cancer cell lines and 31 primary lung cancer tumors for mutations in these genes using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and direct sequencing. Our findings failed to uncover any mutations in these genes. We also examined the expression of ING1 and ING2 in lung cancer cell lines that either had or lacked a p53 mutation, and in a control bronchial epithelium cell line, using quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). ING1 expression was up-regulated in all 7 lung cancer cell lines that had a p53 mutation, while the expression of ING2 was down-regulated in 6 of 7 lung cancer cell lines that had a p53 mutation. These results suggest that the ING1 and ING2 genes have different roles in lung carcinogenesis and progression, and the ING2 gene may be an independent tumor suppressor candidate on p53.