SYNERGISTIC EFFECT OF VASCULAR ENDOTHELIAL GROWTH-FACTOR AND BASIC FIBROBLAST GROWTH-FACTOR ON ANGIOGENESIS IN-VIVO
SYNERGISTIC EFFECT OF VASCULAR ENDOTHELIAL GROWTH-FACTOR AND BASIC FIBROBLAST GROWTH-FACTOR ON ANGIOGENESIS IN-VIVO
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DOI：
10.1161/01.cir.92.9.365
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发表时间：
1995-11-01
期刊：
影响因子：
37.8
通讯作者：
ISNER, JM
中科院分区：
文献类型：
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作者：
ASAHARA, T;BAUTERS, C;ISNER, JM
背景最近的研究表明，血管内皮生长因子和碱性成纤维细胞生长因子在体外诱导血管生成方面可能具有协同作用，因此，我们研究了血管内皮生长因子和碱性成纤维细胞生长因子联合应用对后肢缺血动物模型侧支循环发育有协同作用的假说。方法与结果手术诱导一侧后肢缺血10d后，新西兰大白兔随机分为3组，分别给予血管内皮细胞生长因子500 mU/g(n=6)、碱性成纤维细胞生长因子10 mU/g(n=7)、血管内皮细胞生长因子500 mU/g，然后立即给予10 mU/d碱性成纤维细胞生长因子(n=7)，或仅车辆(对照动物，n=8)，每例均经缺血肢体髂内动脉导管动脉内给药。血管内皮生长因子+碱性成纤维细胞生长因子组第10天的血压比值(BPR，缺血/健肢)为0.82+/-0.01，明显优于对照组(P
Background Recent studies have suggested that vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) may have synergistic effects on the induction of angiogenesis in vitro, Therefore, we investigated the hypothesis that the simultaneous administration of VEGF and bFGF, each having been previously shown to independently enhance collateral development in an animal model of hind limb ischemia, could have a synergistic effect in vivo.Methods and Results Ten days after surgical induction of unilateral hind limb ischemia, New Zealand White rabbits were randomized to receive either VEGF 500 mu g alone (n=6), bFGF 10 mu g alone (n=7), VEGF 500 mu g, immediately followed by 10 mu g bFGF (n=7), or vehicle only (control animals, n=8) in each case administered intra-arterially via a catheter in the internal iliac artery of the ischemic limb. BP ratio (BPR, ischemic/healthy limb) at day 10 for the VEGF+bFGF group was 0.82+/-0.01, much superior (P