DEVELOPMENT OF THE BRAIN-STEM IN THE RAT .4. THYMIDINE-RADIOGRAPHIC STUDY OF THE TIME OF ORIGIN OF NEURONS IN THE PONTINE REGION
DEVELOPMENT OF THE BRAIN-STEM IN THE RAT .4. THYMIDINE-RADIOGRAPHIC STUDY OF THE TIME OF ORIGIN OF NEURONS IN THE PONTINE REGION
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DOI：
10.1002/cne.901940411
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发表时间：
1980-01-01
影响因子：
2.5
通讯作者：
BAYER, SA
中科院分区：
文献类型：
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作者：
ALTMAN, J;BAYER, SA
从妊娠第 12 天和第 13 天 (E12 + 13) 到分娩前一天 (E21 + 22)，各组怀孕大鼠连续每日注射 2 剂 3H-胸苷，以便在胚胎中标记神经元的增殖前体。 60 日龄时，定量测定脑桥区域 14 个细胞核中特定胚胎日生成（或不再标记）的神经元比例。三叉神经中脑核神经元产生高峰时间为E11天或更早，少量在E12天产生。三叉神经运动神经元的产生高峰时间是E12天，有一小部分更早产生。主感觉核的神经元在E13-E16天之间产生，在E14天达到峰值；晚期产生的神经元往往属于一类中型和大型细胞。三叉神经上核和三叉神经下核的大部分神经元在 E15 和 E16 天出现。蓝斑神经元大部分在 E12 天产生，约 20% 的细胞在 E13 天产生。背侧被盖核（Gudden's）的大部分神经元在E13-E15天之间产生，而深部（腹侧）被盖核的大部分神经元在E15天产生。在外侧丘系的背侧核和腹侧核之间也获得了从背侧到尾侧的梯度，前者的神经元在E12-E15天之间产生；后者在 E13-E17 之间。臂旁核外侧部和内侧部的神经元在E13-E15天之间同时产生，在E13天达到峰值。从E11天（或更早）到E15天产生脑桥旁内侧网状结构的神经元的异质集合。小细胞中缝桥的神经元在E13-E15天之间以均匀的速率产生，而中缝大细胞桥的大部分神经元在E15和E16天产生。根据对整个脑干三叉神经复合体的 9 个分区获得的年代测定结果，提出了该系统的细胞遗传学组成部分的假设。背侧和深部被盖核中神经元产生的顺序与它们以先到先服务的方式与乳头核和缰核核分裂的联系有关。
Groups of pregnant rats were injected with 2 successive daily doses of 3H-thymidine from gestational day 12 and 13 (E12 + 13) until the day before parturition (E21 + 22) in order to label in their embryos the proliferating precursors of neurons. At 60 days of age the proportion of neurons generated (or no longer labeled) on specific embryonic days was determined quantitatively in 14 nuclei of the pontine region. Peak production time of neurons of the trigeminal mesencephalic nucleus was on day E11 or earlier, with a small proportion generated on day E12. Peak production time of the trigeminal motor neurons was on day E12, with a small proportion produced earlier. Neurons of the principal sensory nucleus were generated between days E13-E16, with a peak on day E14; the late-produced neurons tended to belong to a class of intermediate and large cells. The bulk of the neurons of the supratrigeminal and infratrigeminal nuclei arose on days E15 and E16. Neurons of the locus coeruleus are produced mostly on day E12, with about 20% of the cells arising on day E13. The bulk of the neurons of the dorsal tegmental nucleus (Gudden''s) are produced between day E13-E15, whereas most of the neurons of the deep (ventral) tegmental nucleus are produced on day E15. A dorsal-to-caudal gradient was also obtained between the dorsal and ventral nuclei of the lateral lemniscus, the neurons of the former being generated between days E12-E15; the latter, between days E13-E17. The neurons of both the pars lateralis and the pars medialis of the parabrachial nucleus were produced simultaneously between days E13-E15, with a peak on day E13. The heterogeneous collection of neurons of the pontine paramedial reticular formation was produced from day E11 (or earlier) until day E15. The neurons of the raphe pontis parvicellularis were generated at an even rate between days E13-E15, whereas the bulk of the neurons of the raphe pontis magnocellularis was produced on days E15 and E16. On the base is of datings obtained for 9 subdivisions of the entire brain stem trigeminal complex, hypotheses were offered of the cytogenetic components of the system. The sequence of neuron production in the dorsal and deep tegmental nuclei was related to their connections with divisions of the mammillary and habenular nuclei on a first come-first serve basis.