Phospholipid association with the bovine cardiac mitochondrial adenosine triphosphatase.
Phospholipid association with the bovine cardiac mitochondrial adenosine triphosphatase.
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磷脂与牛心脏线粒体三磷酸腺苷酶的关联。
DOI：
10.1042/bj2250597
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Cunningham,CC
中科院分区：
文献类型：
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作者：
Brown,RE;Montgomery,RI;Spach,PI;Cunningham,CC
不同磷脂与来自牛心脏线粒体的脂质耗尽的寡霉素敏感性ATP酶的结合[塞拉诺，Kanner & Racker（1976）J.Biol.Chem.251，2453-2461]已经使用三种方法进行了研究。首先，用不同的合成二酰基磷脂重构ATP酶导致ATP酶比活性的2-10倍刺激，这取决于所用的特定磷脂。磷脂头基区域显示以下顺序的ATP酶再活化潜力：二油酰磷脂酰甘油大于二油酰磷脂酸大于二油酰磷脂酰胆碱。此外，与二肉豆蔻酰衍生物相比，当用二油酰磷脂衍生物重构时，ATP酶显示出更高水平的比活性。第二，在纯化后与脂质耗尽的ATP酶相关的剩余磷脂的检查显示存在磷脂酰胆碱、磷脂酰乙醇胺和二磷脂酰甘油。没有任何这些磷脂的相对富集（与它们在亚线粒体颗粒中的分布相比）。因此，在线粒体ATP酶中没有发现ATP酶与任何一种磷脂之间的优先关联。第三，采用胆酸钠介导的磷脂交换程序研究ATP酶的磷脂需求。利用合成磷脂酸或磷脂酰胆碱，可以实现用脂质耗尽的ATP酶替换约50%的剩余线粒体磷脂。检查的位移线粒体磷脂显示，磷脂酰胆碱，磷脂酰乙醇胺，和二磷脂酰甘油被替换为同等设施。
The association of different phospholipids with a lipid-depleted oligomycin-sensitive ATPase from bovine cardiac mitochondria [Serrano, Kanner & Racker (1976) J. Biol. Chem. 251, 2453-2461] has been examined using three approaches. First, reconstitution of the ATPase with different synthetic diacyl phospholipids resulted in a 2-10-fold stimulation of ATPase specific activity depending upon the particular phospholipid employed. The phospholipid headgroup region displayed the following order of ATPase reactivation potential: dioleoylphosphatidylglycerol greater than dioleoylphosphatidic acid greater than dioleoylphosphatidylcholine. Furthermore, the ATPase showed higher levels of specific activity when reconstituted with dioleoyl phospholipid derivatives compared with dimyristoyl derivatives. Second, examination of the phospholipid remaining associated with the lipid-depleted ATPase upon purification showed that phosphatidylcholine, phosphatidylethanolamine, and diphosphatidylglycerol were present. No relative enrichment of any of these phospholipids (compared with their distribution in submitochondrial particles) was noted. Therefore, no preferential association between the ATPase and any one phospholipid could be found in the mitochondrial ATPase. Third, the sodium cholate-mediated phospholipid exchange procedure was employed for studying the phospholipid requirements of the ATPase. Replacement of about 50% of the mitochondrial phospholipid remaining with the lipid-depleted ATPase could be achieved utilizing either synthetic phosphatidic acid or phosphatidylcholine. Examination of the displaced mitochondrial phospholipid showed that phosphatidylcholine, phosphatidylethanolamine, and diphosphatidylglycerol were replaced with equal facility.