CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs
CIDE gene expression in adipose tissue, liver, and skeletal muscle from obese and lean pigs
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肥胖猪和瘦猪的脂肪组织、肝脏和骨骼肌中的 CIDE 基因表达
DOI：
10.1631/jzus.b1600294
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发表时间：
2017-06-01
影响因子：
5.1
通讯作者：
Jiang, Zong-yong
中科院分区：
文献类型：
--
作者：
Qiu, Yue-qin;Yang, Xue-fen;Jiang, Zong-yong
在肥胖的小鼠和人类模型中，细胞死亡诱导DNA碎片因子α样效应器(CIDE)家族包括CideB和Cidec的表达显著增加。然而，关于这些基因在猪身上表达的信息较少。在此，我们假设瘦肉型(DurocxLandracexyorkshire，DLY)猪和肥胖型(兰塘)猪的不同脂肪积累归因于猪CIDE调节脂肪代谢。结果表明，CideB和Cideb在脂肪组织中高表达，在骨骼肌中表达相对较高，而Cideb主要在肝脏中表达。兰塘猪的白色脂肪和骨骼肌Cideb和Cidec基因丰度高于DLY猪，肝脏和肌肉Cideb基因丰度高于DLY猪。脂代谢相关基因包括固醇调节元件结合蛋白1c(SREBP-1c)、肝细胞核因子-4α(HNF-4α)、过氧化物酶体增殖物激活受体-1α(PGC-1α)、脂肪酸合成酶(FASN)、二酰甘油O-酰基转移酶1(DGAT1)和DGAT2在肥胖猪脂肪组织中的表达水平高于瘦肉猪。与DLY猪相比，蓝塘猪肝脏SREBP-1c、HNF-4α和PGC-1α的mRNA丰度较高，骨骼肌SREBP-1c、HNF-4α、PGC-1α和DGAT2的表达较高。然而，肥胖猪的脂肪组织、肝脏和骨骼肌中的perlipin2mRNA水平显著低于瘦肉猪。此外，肥胖猪的血浆非酯化脂肪酸(NEFA)、葡萄糖和三酰甘油(TAG)水平高于瘦肉猪。相关分析进一步发现，CIDE基因的表达与两个品种的背脂厚度(BFT)、腹脂质量(AFM)、NEFA、TAG和血糖水平呈正相关。综上所述，这些数据表明，杀猪药可能调节了脂肪代谢，并促进了兰塘猪脂肪沉积和肥胖的发展。
The expression of the cell death-inducing DNA fragmentation factor alpha-like effector (CIDE) family including Cidea, Cideb, and Cidec was significantly increased in mouse and human models of obesity. However, there was less information on these genes' expression in pigs. Here, we hypothesized that different fat accumulation between lean (DurocxLandracexYorkshire gilts, DLY) and obese (Lantang) pigs was attributed to porcine CIDE-modulating lipid metabolism. Our data showed that Cidea and Cidec were expressed at a high level in adipose tissue, and at a relatively high level in skeletal muscle, whereas Cideb was mainly expressed in the liver in both breeds of pig. Lantang pigs had higher white adipose and skeletal muscle Cidea and Cidec mRNA abundance, and hepatic and muscle Cideb mRNA than DLY pigs. Lipid metabolism-related genes including sterol regulatory element binding protein 1c (SREBP-1c), hepatocyte nuclear factor-4 alpha (HNF-4 alpha), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1 alpha), fatty acid synthase (FASN), diacylglycerol O-acyltransferase 1 (DGAT1), and DGAT2 showed a higher expression level in adipose tissue from obese pigs than in that from lean pigs. Lantang pigs exhibited higher mRNA abundance for liver SREBP-1c, HNF-4 alpha, and PGC-1 alpha, and higher skeletal muscle SREBP-1c, HNF-4 alpha, PGC-1 alpha, and DGAT2 expression, as compared with DLY pigs. However, the perlipin2 mRNA levels in adipose tissues, liver, and skeletal muscle were significantly lower in obese pigs than in their lean counterparts. Furthermore, plasma non-esterified fatty acid (NEFA), glucose, and triacylglycerol (TAG) levels were greater in obese pigs than in lean pigs. Finally, data from correlation analysis further found that CIDE mRNA expression was positively correlated with back fat thickness (BFT), abdominal fat mass (AFM), and the levels of NEFA, TAG, and glucose in the two breeds. Collectively, these data revealed that the porcine CIDEs possibly modulated lipid metabolism and contributed to the development of fat deposition and obesity in Lantang pigs.