Expression of PprI from Deinococcus radiodurans Improves Lactic Acid Production and Stress Tolerance in Lactococcus lactis.
Expression of PprI from Deinococcus radiodurans Improves Lactic Acid Production and Stress Tolerance in Lactococcus lactis.
复制标题
耐辐射奇球菌中 PprI 的表达可提高乳酸乳球菌的乳酸产量和应激耐受性
DOI：
10.1371/journal.pone.0142918
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hua Y
中科院分区：
文献类型：
--
作者：
Dong X;Tian B;Dai S;Li T;Guo L;Tan Z;Jiao Z;Jin Q;Wang Y;Hua Y
PprI 是一种通用开关蛋白，可调节嗜极菌耐辐射球菌细胞耐药途径中涉及的某些蛋白质的表达。在本研究中，我们使用乳球菌穿梭载体pMG36e将pprI转化到乳酸乳球菌菌株MG1363中，并研究了其在胁迫下对乳酸乳球菌的耐受性和乳酸产生的影响。通过蛋白质印迹测定证实，PprI 在乳酸乳球菌中稳定表达。表达 PprI 的乳酸乳球菌表现出显着改善的氧化应激和高渗透压抵抗力。这种增强的细胞对应激源的耐受性可能是由于 pprI 对抗性相关基因（例如，recA、recO、sodA 和 nah）的调节所致。此外，转化的乳酸乳球菌表现出乳酸产量增加，这归因于乳酸脱氢酶活性的增强。这些结果表明pprI可以提高乳酸乳球菌对环境胁迫的耐受性，并且该转化菌株是乳酸生产工业应用的有希望的候选者。
PprI is a general switch protein that regulates the expression of certain proteins involved in pathways of cellular resistance in the extremophilic bacterium Deinococcus radiodurans. In this study, we transformed pprI into Lactococcus lactis strain MG1363 using the lactococcal shuttle vector pMG36e and investigated its effects on the tolerance and lactic acid production of L. lactis while under stress. PprI was stably expressed in L. lactis as confirmed by western blot assays. L. lactis expressing PprI exhibited significantly improved resistance to oxidative stress and high osmotic pressure. This enhanced cellular tolerance to stressors might be due to the regulation of resistance-related genes (e.g., recA, recO, sodA, and nah) by pprI. Moreover, transformed L. lactis demonstrated increased lactic acid production, attributed to enhanced lactate dehydrogenase activity. These results suggest that pprI can improve the tolerance of L. lactis to environmental stresses, and this transformed bacterial strain is a promising candidate for industrial applications of lactic acid production.