Facile Cholesterol Loading with a New Probe ezFlux Allows for Streamlined Cholesterol Efflux Assays.
Facile Cholesterol Loading with a New Probe ezFlux Allows for Streamlined Cholesterol Efflux Assays.
复制标题
使用新探针 ezFlux 轻松加载胆固醇，可简化胆固醇流出测定。
DOI：
10.1021/acsomega.0c03112
复制
发表时间：
2020
期刊：
影响因子：
4.1
通讯作者：
Maiseyeu,Andrei
中科院分区：
文献类型：
--
作者：
Ravodina,AnastasiaM;Badgeley,MarcusA;Rajagopalan,Sanjay;Fedyukina,DariaV;Maiseyeu,Andrei
在这里，我们报告了一种基于纳米颗粒的探针，它可以在胆固醇流出（CE）测定中用胆固醇轻松标记细胞。这种探针称为ezFlux，通过筛选用Fo Prster共振能量转移（FRET）报告基因工程化的多种纳米制剂进行了优化。通过测试巨噬细胞中的摄取、细胞内降解途径和CE测定中的性能，证实了ezFlux与标准半合成乙酰化低密度脂蛋白（acLDL）的理化和生物相似性。一步快速自组装制造使ezFlux成为acLDL的有吸引力的替代品。我们还表明，使用ezFlux的CE测试比使用商业试剂盒或acLDL进行的测试便宜得多。此外，我们分析了测量CE的临床试验，并表明ezFlux在全球许多使用CE评估细胞和脂蛋白功能的研究和临床实验室中占有一席之地。
Here, we report a nanoparticle-based probe that affords facile cell labeling with cholesterol in cholesterol efflux (CE) assays. This probe, called ezFlux, was optimized through a screening of multiple nanoformulations engineered with a Förster resonance energy transfer (FRET) reporter. The physicochemical- and bio-similarity of ezFlux to standard semi-synthetic acetylated low-density lipoprotein (acLDL) was confirmed by testing uptake in macrophages, the intracellular route of degradation, and performance in CE assays. A single-step fast self-assembly fabrication makes ezFlux an attractive alternative to acLDL. We also show that CE testing using ezFlux is significantly cheaper than that performed using commercial kits or acLDL. Additionally, we analyze clinical trials that measure CE and show that ezFlux has a place in many research and clinical laboratories worldwide that use CE to assess cellular and lipoprotein function.