Does genetic testing for obesity influence confidence in the ability to lose weight? A pilot investigation
Does genetic testing for obesity influence confidence in the ability to lose weight? A pilot investigation
复制标题
DOI：
10.1016/s0002-8223(01)00323-6
复制
发表时间：
2001-11-01
影响因子：
--
通讯作者：
Poehlman, ET
中科院分区：
文献类型：
--
作者：
Harvey-Berino, J;Gold, EC;Poehlman, ET
方法本研究是一项大型研究的一部分（9,10），旨在测试肥胖携带者和非b3AR基因变异携带者之间的生理和代谢差异。由于50岁以上女性的肥胖患病率增加（高达64.4%）（11），绝经后妇女成为本研究的目标。佛蒙特州伯灵顿地区的肥胖、白人、绝经后妇女通过当地广告招募。纳入标准为停止月经至少1年，身体质量指数（BMI）大于28 kg/[m]。[Sup . 2]和缺乏运动。女性还必须不吸烟，不接受激素替代疗法，非糖尿病患者。在受试者知情同意后进行b3AR变异的筛查。这项研究得到了佛蒙特大学人类研究和医学科学委员会的批准。3-肾上腺素受体基因b3AR变异的基因分型方法如前所述（12），采用pgr限制性片段长度多态性分析。
METHODSThis study was part of a larger study (9, 10) designed to test physiological and metabolic differences between obese carriers and noncarriers of the b3AR gene variant. Because of the increased prevalence of obesity in women older than 50 years of age (as high as 64.4%)(11), postmenopausal women were targeted for this study. Obese, white, postmenopausal women in the greater Burlington, Vermont, area were recruited by local advertisement. Inclusion criteria were the cessation of menstruation for at least 1 year, a body mass index (BMI) greater than 28 kg/[m. sup. 2], and physical inactivity. Women also had to be nonsmokers, not taking hormone replacement therapy, and nondiabetic. Screening for the presence of the b3AR variant was performed after subjects gave their informed consent. This study was approved by the Committee on Human Research and Medical Sciences of the University of Vermont. Genotyping for the b3AR variant in the 3-adrenoreceptor gene was performed as previously described (12) by PGR-restriction fragment length polymorphism analysis.