Birth of mice from vitrified/warmed 2-cell embryos transported at a cold temperature
Birth of mice from vitrified/warmed 2-cell embryos transported at a cold temperature
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DOI：
10.1016/j.cryobiol.2008.12.011
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发表时间：
2009-04-01
期刊：
影响因子：
2.7
通讯作者：
Nakagata, Naomi
中科院分区：
文献类型：
--
作者：
Takeo, Toru;Kaneko, Takehito;Nakagata, Naomi
2-细胞胚胎冷冻保存是保存基因工程小鼠品系的有效技术。基因工程小鼠在实验室之间的运输经常使用这种冷冻保存的2-细胞胚胎。然而，接收实验室需要熟练的技能和特殊的仪器才能从冷冻保存和运输的胚胎中获得活的幼崽。因此，在本研究中，我们试图解决玻璃化冷冻/加温2-细胞胚胎在低温下的储存和运输问题。在冷藏实验中，M2液中保存24、48和72h的2-细胞胚胎的囊胚率较高(分别为83%、63%和43%)。2-细胞胚胎在PB1和MWM中保存24 h后仍保持发育潜能，但48h后胚胎发育率显著下降至低水平(PB1：0%；MWM：5%)。在移植实验中，从所有接受实验室的玻璃化/加温2-细胞胚胎中获得了许多幼崽(发育成功率：49%；249/511)。综上所述，玻璃化冷冻/升温的2-细胞胚胎在M2培养液中的低温短期储存和运输可以保持它们发育成活体幼崽的能力。(C)2008 Elsevier Inc.保留所有权利。
Cryopreservation of 2-cell embryos is an effective technology for storage of genetically engineered mouse strains. Transport of genetically engineered mice between laboratories has frequently been performed using such cryopreserved 2-cell embryos. However, the receiving laboratory requires proficient skills and special instruments to obtain live young from cryopreserved and transported embryos. Therefore, in this study, we tried to address the storage and transport of vitrified/warmed 2-cell embryos at a cold temperature. In cold storage experiments, the development rates of 2-cell embryos stored in M2 medium for 24,48 and 72 h into blastocysts were relatively high (83%, 63% and 43%, respectively). Although, 2-cell embryos stored in PB1 and mWM maintained the developmental potency for 24 h, the rates were markedly decreased to low levels after 48 h (PB1: 0%; mWM: 5%). In transport experiments, many pups were obtained from vitrified/warmed 2-cell embryos transported at a cold temperature in all receiving laboratories (incidence of successful development: 49%; 249/511). In summary, short-term storage and transport of vitrified/warmed 2-cell embryos in M2 medium at a cold temperature can maintain their ability to develop into live young. (C) 2008 Elsevier Inc. All rights reserved.