Selective enrichment of N-linked glycopeptides and glycans by using a dextran-modified hydrophilic material
Selective enrichment of N-linked glycopeptides and glycans by using a dextran-modified hydrophilic material
复制标题
使用葡聚糖修饰的亲水材料选择性富集 N-连接糖肽和聚糖
DOI：
10.1002/jssc.201700995
复制
发表时间：
2018-05-01
影响因子：
3.1
通讯作者：
Liang, Xinmiao
中科院分区：
文献类型：
--
作者：
Chen, Linlin;Ding, Di;Liang, Xinmiao
由于糖肽的丰度相对较低、聚糖的结构多样性和共存基质，利用基于质谱的方法对来自生物来源的蛋白质进行糖基化分析具有挑战性。在这项研究中，引入了定制的葡聚糖键合硅胶固定相，用于选择性富集复杂生物样品中的糖肽和聚糖。该材料在糖蛋白质组学评价中表现出优于商业琼脂糖凝胶的选择性和更广泛的糖基化位点覆盖。此外，胎球蛋白、α（1）-酸性糖蛋白和人血清N-糖组的糖组学分析也表明，葡聚糖键合的二氧化硅的灵敏度、选择性和糖型覆盖率高于琼脂糖凝胶和多孔石墨化碳。因此，葡聚糖键合二氧化硅有望在糖蛋白质组学和糖组学领域做出贡献。
Glycosylation analysis of proteins from biological sources utilizing mass spectrometry based approaches is challenging due to the relatively low abundance of glycopeptides, the structural diversity of glycans, and the coexisting matrices. In this study, a customized dextran-bonded silica-based stationary phase was introduced for selective enrichment of glycopeptides and glycans from complex biological samples. This material has exhibited superior selectivity and broader glycosylation site coverage over commercial Sepharose in glycoproteomic evaluation. Additionally, the glycomic analysis of fetuin, alpha(1)-acid glycoprotein, and human serum N-glycome also indicated the relatively higher sensitivity, selectivity, and glycoform coverage of dextran-bonded silica than that of Sepharose and porous graphitized carbon. Therefore, the dextran-bonded silica is expected to make contributions in the fields of glycoproteomics and glycomics.