Skin Carotenoids: A Biomarker of Fruit and Vegetable Intake in Children
Skin Carotenoids: A Biomarker of Fruit and Vegetable Intake in Children
复制标题
DOI：
10.1016/j.jand.2014.04.026
复制
发表时间：
2014-08-01
影响因子：
4.8
通讯作者：
Gast, Julie
中科院分区：
文献类型：
--
作者：
Aguilar, Sheryl S.;Wengreen, Heidi J.;Gast, Julie
背景对成年受试者的研究发现，血清类胡萝卜素和皮肤类胡萝卜素之间有很强的相关性，这是通过共振拉曼光谱（RRS）测量的。没有发表的研究已经检查了皮肤和血清类胡萝卜素之间的相关性internations.Objectives我们的目的是验证皮肤RRS方法对血清类胡萝卜素测量的高效液相色谱法，并确定RRS是否可以作为一个有效的生物标志物的水果和蔬菜（F/V）摄入internations.Design在我们的横断面研究，参与者是45名5至17岁的健康儿童，他们在4周的时间内提供了用于评估血清类胡萝卜素浓度的三个血液样本和手掌的三个RRS皮肤测量值。在4周内使用27项食物频率问卷（FFQ）和自动多遍24小时每日回忆对F/V的饮食摄入进行了三次评估。对间隔至少7天完成的三个FFQ的摄入量估计值取平均值。结果皮肤类胡萝卜素与血清类胡萝卜素水平高度相关（R-2=0.62; P
Background Studies of adult subjects have found a strong correlation between serum carotenoids and skin carotenoids measured by resonance Raman spectroscopy (RRS). No published studies have examined correlations between skin and serum carotenoids among children.Objectives We aimed to validate skin RRS methodology against serum carotenoid measurements by high-performance liquid chromatography and to determine whether RRS can be used as a valid biomarker of fruit and vegetable (F/V) intake among children.Design In our cross-sectional study, participants were 45 healthy children aged 5 to 17 years who provided three blood samples used to assess serum carotenoid concentrations and three RRS skin measurements of the palm within a 4-week period. Dietary intake of F/V was assessed three times within 4 weeks using a 27-item food frequency questionnaire (FFQ) and an automated multiple-pass 24-hour daily recall. Estimates of intake from three FFQs, completed at least 7 days apart, were averaged. Estimates of intake from 24-hour daily recalls were collected on 2 weekdays and 1 weekend day and averaged.Results Levels of skin and, serum carotenoids were highly correlated (R-2=0.62; P