The effect of detachment of the articular cartilage from its calcified zone on the cartilage microstructure, assessed by 2H-spectroscopic double quantum filtered MRI
The effect of detachment of the articular cartilage from its calcified zone on the cartilage microstructure, assessed by 2H-spectroscopic double quantum filtered MRI
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DOI：
10.1016/j.orthres.2004.06.005
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Navon, G
中科院分区：
文献类型：
--
作者：
Keinan-Adamsky, K;Shinar, H;Navon, G
大多数关于关节软骨特性的研究都是在软骨从骨中分离后进行的。在目前的工作中，我们研究了脱离对胶原纤维结构的影响。我们使用一维H-2双量子滤波MRI的软骨骨栓平衡在氘盐水。在不同区域观察到的四极分裂与胶原纤维的有序程度和密度有关。该方法是非破坏性的，允许在同一个插头上进行测量，而无需固定。脱水、切片和脱钙。从钙化区的桡骨分离导致软骨塞在生理盐水中肿胀，并伴随着四极分裂的减少。将机械压力对分离软骨和钙化区-骨栓的H-2四极分裂的影响与完整软骨-骨栓中相同区域的H-2四极分裂进行比较。与完整的软骨-骨塞相比，在小得多的载荷下，分离软骨的径向区域中的分裂塌陷。载荷对软骨大小的影响对于分离的塞子也更大。这些结果表明，通过钙化区将软骨锚定到骨在保持胶原纤维的有序性方面起着重要作用。完整和蛋白聚糖耗尽的软骨中的水H-2四极分裂是相同的。这表明蛋白聚糖对胶原纤维的有序化没有贡献。(C)2004骨科研究学会。由爱思唯尔有限公司出版。保留所有权利。
Most studies on articular cartilage properties have been conducted after detachment of the cartilage from the bone. In the present work we investigated the effect of detachment on collagen fiber architecture. We used one-dimensional H-2 double quantum filtered MRI on cartilage bone plugs equilibrated in deuterated saline. The quadrupolar splitting observed in the different zones were related to the degree of order and the density of the collagen fibers. The method is non-destructive, allowing for measurements on the same plug without the need for fixation. dehydration, sectioning and decalcification. Detachment of the radial from the calcified zone resulted in swelling of the cartilage plug in physiological saline and a concomitant decrease in the quadrupolar splitting. The effect of mechanical pressure on the H-2 quadrupolar splittings for the detached cartilage and for the calcified zone-bone plugs were compared with those of the same zones in the intact cartilage-bone plug. The splitting in the radial zone of the detached cartilage collapsed at Much smaller loads compared to the intact cartilage-bone plug. The effect of the load on the size of the cartilage was also greater for the detached plug. These results indicate that anchoring of the cartilage to the bone through the calcified zone plays an important role in retaining the order of the collagen fibers. The water H-2 quadrupolar splitting in intact and proteoglycan-depleted cartilage was the same. indicating that the proteoglycans do not contribute to the ordering of the collagen fibers. (C) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.