Resistance of spores of Bacillus species to ultraviolet light
Resistance of spores of Bacillus species to ultraviolet light
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DOI：
10.1002/em.1058
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发表时间：
2001-01-01
影响因子：
2.8
通讯作者：
Setlow, P
中科院分区：
文献类型：
--
作者：
Setlow, P
包括B.枯草芽孢杆菌，是5至50倍更耐紫外线辐射比相应的生长细胞。这种提高的孢子UV抗性是由于：a）孢子内DNA的光化学，因为UV产生很少的环丁烷二聚体（如果有的话），而是称为孢子光产物（SP; 5-胸腺嘧啶-5，6-二氢胸腺嘧啶）的光产物（图1）;和B）孢子萌发期间的DNA修复，特别是SP特异性修复。孢子DNA的新的UV光化学很大程度上是由于其与一组小的酸溶性蛋白质（SASP）的饱和，这是孢子所特有的，其结合改变了DNA构象，从而改变了其光化学。SP特异性修复也是孢子所特有的，并且通过不依赖于光照的SP裂解酶进行，SP裂解酶是一种铁硫蛋白，其利用S-腺苷甲硫氨酸催化SP单体化而无需DNA主链裂解。Environ.摩尔变异体(C)2001 Wiley-Liss，Inc.
Dormant spores of the various Bacillus species, including B. subtilis, are 5 to 50 times more resistant to UV radiation than are the corresponding growing cells. This elevated spore UV resistance is due to: a) the photochemistry of DNA within spores, as UV generates few if any cyclobutane dimers, but rather a photoproduct (Fig. 1) called spore photoproduct (SP; 5-thyminyl-5,6-dihydrothymine); and b) DNA repair, in particular SP-specific repair, during spore germination. The novel UV photochemistry of spore DNA is largely due to its saturation with a group of small, acid-soluble proteins (SASP), which are unique to spores and whose binding alters the DNA conformation and thus its photochemistry. SP-specific repair is also unique to spores and is carried out by alight-independent SP-lyase, an iron-sulfur protein that utilizes S-adenosylmethionine to catalyze SP monomerization without DNA backbone cleavage. Environ. Mol. Mutagen. (C) 2001 Wiley-Liss, Inc.