Exploring urine:serum fractional excretion ratios as potential biomarkers for lupus nephritis.
Exploring urine:serum fractional excretion ratios as potential biomarkers for lupus nephritis.
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DOI：
10.3389/fimmu.2022.910993
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
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作者：
这项探索性研究的目的是确定在系统性红斑狼疮（SLE）活动性肾脏疾病的识别中，尿：血清排泄分数比是否优于相应的尿生物标志物蛋白。采用ELISA法检测36例SLE患者和12例健康对照者血清和尿液中ALCAM、钙蛋白酶抑制素、血红素结合蛋白、过氧化物酶6（PRDX 6）、血小板因子4（PF 4）、备解素、TFPI和VCAM-1的水平。将尿液和血清生物标志物水平相对于肌酐标准化后，计算分析的生物标志物的排泄分数。进一步验证队列的50例SLE患者被纳入验证的初步结果。所有8种蛋白质的FE比率在鉴定肾脏疾病活动性方面优于常规疾病活动性标志物，如抗dsDNA、C3和C4。除VCAM-1FE外，其他指标在区分LN活性方面均上级相应的尿液生物标志物水平，并与肾脏SLEDAI呈正相关。ALCAMFE、PF 4FE和备解素FE比值在区分活动性LN和非活动性SLE中表现出最高的准确性（AUC>0.9）。四个FE比率显示出完美的灵敏度（calpastatin、PRDX 6、PF 4和备解素），而ALCAMFE、PF 4FE和备解素FE显示出对活动性LN的最高特异性值。此外，这些新型生物标志物中的几种与较高的肾脏病理活动指数相关。在验证队列中，ALCAMFE、PF 4FE和备解素FE再次表现出更高的准确性指标，超过相应的尿液和血清生物标志物水平，其中ALCAMFE在区分活动性LN与非活动性SLE方面表现出95%的准确性。对于大多数检测的蛋白质，尿：血清排泄分数比在识别SLE活动性肾脏受累方面优于相应的尿和血清蛋白测量。因此，这类新的SLE生物标志物应该在更大的独立队列中进行系统评价，以评估其在LN评估中的诊断效用。
The goal of this exploratory study is to determine if urine:serum fractional excretion ratios can outperform the corresponding urinary biomarker proteins in identifying active renal disease in systemic lupus erythematosus (SLE). Thirty-six adult SLE patients and twelve healthy controls were examined for serum and urine levels of 8 protein markers, namely ALCAM, calpastatin, hemopexin, peroxiredoxin 6 (PRDX6), platelet factor 4 (PF4), properdin, TFPI and VCAM-1, by ELISA. Fractional excretion of analyzed biomarkers was calculated after normalizing both the urine and serum biomarker levels against creatinine. A further validation cohort of fifty SLE patients was included to validate the initial findings. The FE ratios of all 8 proteins interrogated outperformed conventional disease activity markers such as anti-dsDNA, C3 and C4 in identifying renal disease activity. All but VCAM-1FE were superior to the corresponding urine biomarkers levels in differentiating LN activity, exhibiting positive correlation with renal SLEDAI. ALCAMFE, PF4FE and properdinFE ratios exhibited the highest accuracy (AUC>0.9) in distinguishing active LN from inactive SLE. Four of the FE ratios exhibited perfect sensitivity (calpastatin, PRDX6, PF4 and properdin), while ALCAMFE, PF4FE and properdinFE exhibited the highest specificity values for active LN. In addition, several of these novel biomarkers were associated with higher renal pathology activity indices. In the validation cohort ALCAMFE, PF4FE and properdinFE once again exhibited higher accuracy metrics, surpassing corresponding urine and serum biomarkers levels, with ALCAMFE exhibiting 95% accuracy in distinguishing active LN from inactive SLE. With most of the tested proteins, urine:serum fractional excretion ratios outperformed corresponding urine and serum protein measurements in identifying active renal involvement in SLE. Hence, this novel class of biomarkers in SLE ought to be systemically evaluated in larger independent cohorts for their diagnostic utility in LN assessment.
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影响因子：
4.9
作者：
Landolt-Marticorena C;Prokopec SD;Morrison S;Noamani B;Bonilla D;Reich H;Scholey J;Avila-Casado C;Fortin PR;Boutros PC;Wither J
通讯作者：
Wither J
影响因子：
5.7
作者：
Bikfalvi, A
通讯作者：
Bikfalvi, A
DOI：
10.1172/jci147334
发表时间：
2022-01-04
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Chalmers SA;Ayilam Ramachandran R;Garcia SJ;Der E;Herlitz L;Ampudia J;Chu D;Jordan N;Zhang T;Parodis I;Gunnarsson I;Ding H;Shen N;Petri M;Mok CC;Saxena R;Polu KR;Connelly S;Ng CT;Mohan C;Putterman C
通讯作者：
Putterman C
影响因子：
4.9
作者：
Mok CC;Soliman S;Ho LY;Mohamed FA;Mohamed FI;Mohan C
通讯作者：
Mohan C
影响因子：
13.3
作者：
Aringer, Martin;Costenbader, Karen;Johnson, Sindhu R.
通讯作者：
Johnson, Sindhu R.