The impact of cesarean delivery on infant DNA methylation.
The impact of cesarean delivery on infant DNA methylation.
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剖宫产对婴儿DNA甲基化的影响
DOI：
10.1186/s12884-021-03748-y
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发表时间：
2021-03-30
影响因子：
3.1
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Chen Q;Ming Y;Gan Y;Huang L;Zhao Y;Wang X;Liu Y;Zhang J
背景越来越多的证据表明，剖腹产可能对婴儿的健康有长期的影响。但潜在的机制仍不清楚。本研究旨在探讨是否剖宫产产妇要求无任何医疗指征（CDMR）的影响DNA甲基化状态在脐带血中的infant.MethodsA横断面研究在中国上海进行。2012年共招募了70例CDMR和70例阴道分娩（VD）。应用Illumina Infinium Human Methylation 450 K BeadChip检测30例CDMR和30例VD新生儿脐血DNA甲基化状态。为了验证结果，使用靶向亚硫酸氢盐测序法检测另外40例CDMR和40例VD新生儿的脐带血DNA甲基化状态。共有497个CpG位点，从40个基因被包括在analysis.ResultsA共165差异甲基化的位置（DMPs）表现出差异的DNA甲基化的10%或以上的CDMR和VD组之间，其中许多是有关的免疫系统的发展。靶向亚硫酸氢盐测序结果显示，CDMR组有16个基因（16/22，72.7%）甲基化水平高于VD组。其中，5个基因与免疫系统相关。考虑到估计的细胞类型的比例后，有几个显着差异CDMR和VD groups.ConclusionsThe DMPs之间确定CDMR和VD组可能在很大程度上解释了细胞类型的比例之间的DNA甲基化。需要进一步的研究来分别检查每种细胞类型中的DNA甲基化。
BackgroundMounting evidence suggests that cesarean delivery may have a long-lasting effect on infant health. But the underlying mechanisms remain unclear. This study aims to examine whether cesarean delivery on maternal request without any medical indications (CDMR) impacts DNA methylation status in the umbilical cord blood of the infant.MethodsA cross-sectional study was conducted in Shanghai, China. A total of 70 CDMR and 70 vaginal deliveries (VD) were recruited in 2012. The cord blood DNA methylation status was measured in 30 CDMR and 30 VD newborns using Illumina Infinium Human Methylation 450 K BeadChip. To validate the results, the cord blood DNA methylation status was measured in another 40 CDMR and 40 VD newborns using targeted bisulfite sequencing assay. A total of 497 CpG sites from 40 genes were included in the analysis.ResultsA total of 165 differentially methylated positions (DMPs) exhibited differences in DNA methylation by 10% or more between the CDMR and VD groups, many of which were related to the development of the immune system. Based on the targeted bisulfite sequencing assay, 16 genes (16/22, 72.7%) had higher methylation level in the CDMR group than the VD group. Among them, 5 genes were related to the immune system. After considering the estimation of cell type proportions, there was few significant differences in DNA methylation between CDMR and VD groups.ConclusionsThe DMPs identified between CDMR and VD groups might be largely explained by the cell type proportions. Further studies are needed to examine DNA methylation in each cell type separately.