Phytophthora infestans sporangia produced in artificial media and plant lesions have subtly divergent transcription profiles but equivalent infection potential and aggressiveness
Phytophthora infestans sporangia produced in artificial media and plant lesions have subtly divergent transcription profiles but equivalent infection potential and aggressiveness
复制标题
人工培养基和植物病斑中产生的致病疫霉孢子囊的转录谱略有不同，但感染潜力和攻击性相当
DOI：
10.1094/mpmi-12-18-0349-ta
复制
发表时间：
2019
影响因子：
3.5
通讯作者：
Judelson, Howard
中科院分区：
文献类型：
--
作者：
Leesutthiphonchai, Wiphawee;Judelson, Howard
马铃薯晚疫病病原体致病疫霉的孢子囊经常用于病原体生物学和植物对感染的反应的研究。对卵菌的孢子生物学研究可能具有挑战性，因为它们的孢子囊具有生理活性，并会随着环境因素和衰老而发生变化。人工培养基中的孢子囊和植物病斑中的孢子囊在功能上是否相同一直是一个争论的话题。为了解决这些问题，我们比较了黑麦蔗糖培养基、马铃薯和番茄小叶以及马铃薯块茎的孢子囊的转录组和感染能力。所有来源的孢子囊 mRNA 谱之间都观察到微小差异，包括编码代谢酶、细胞壁降解酶和 ABC 转运蛋白的基因的变异。孢子囊年龄的微小差异也导致转录组的变化。小心地使用相似成熟度的孢子囊，我们观察到来自培养基或植物病灶的孢子囊具有相似的游动孢子释放和孢囊萌发率。根据单孢子接种测定，传单的感染率或攻击性也没有差异。这些结果与该杂志最近发表的结果不一致。尽管如此，我们得出的结论是，植物和培养基培养的孢子囊在功能上相似，并强调在孢子囊实验中使用“最佳实践”以获得可靠结果的重要性。
Sporangia of the potato late blight agentPhytophthora infestansare often used in studies of pathogen biology and plant responses to infection. Investigations of spore biology can be challenging in oomycetes because their sporangia are physiologically active and change in response to environmental factors and aging. Whether sporangia from artificial media and plant lesions are functionally equivalent has been a topic of debate. To address these issues, we compared the transcriptomes and infection ability of sporangia from rye-sucrose media, potato and tomato leaflets, and potato tubers. Small differences were observed between the mRNA profiles of sporangia from all sources, including variation in genes encoding metabolic enzymes, cell-wall-degrading enzymes, and ABC transporters. Small differences in sporangia age also resulted in variation in the transcriptome. Taking care to use sporangia of similar maturity, we observed that those sourced from media or plant lesions had similar rates of zoospore release and cyst germination. There were also no differences in infection rates or aggressiveness on leaflets, based on single-spore inoculation assays. Such results are discordant with those of a recent publication in this journal. Nevertheless, we conclude that sporangia from plant and media cultures are functionally similar and emphasize the importance of using “best practices” in experiments with sporangia to obtain reliable results.