A recyclable electrochemical sensing platform for breast cancer diagnosis based on homogeneous DNA hybridization and host-guest interaction between cucurbit [7]uril and ferrocene-nanosphere with signal amplification
A recyclable electrochemical sensing platform for breast cancer diagnosis based on homogeneous DNA hybridization and host-guest interaction between cucurbit [7]uril and ferrocene-nanosphere with signal amplification
复制标题
用于乳腺癌诊断的可回收电化学传感平台，基于葫芦[7]脲和二茂铁纳米球之间的均质 DNA 杂交和主客体相互作用，并具有信号放大功能
DOI：
10.1016/j.jelechem.2016.11.027
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发表时间：
2016-12-15
影响因子：
4.5
通讯作者：
He, Pingang
中科院分区：
文献类型：
--
作者：
Yang, Shuai;You, Min;He, Pingang
乳腺癌易感基因（BRCA）与乳腺癌的发生密切相关，近年来，研究者采用多种方法对BRCA进行了追踪。本工作基于BRCA DNA与二茂铁标记的DNA/Au nanosphere（FcNS）和辣根过氧化物酶标记的DNA/Au nanosphere（HRPNS）串联体的均相杂交，以及吸附在电极上的葫芦[7]脲（CB [7]）与杂交复合物上的Fc之间的主客体相互作用，构建了一种新型的可回收电化学传感平台，用于检测BRCA DNA。随着实验条件的优化，所制备的传感平台显示出提高的灵敏度，具有25 pM（S/N = 3）的低检测限，这归因于使用FcNS和HRPNS多联体的信号的双重放大，以及对BRCA DNA的高特异性。随着pH值的升高，络合物从CB [7]修饰电极上解离，回收利用传感平台。该检测策略在人血清样品分析中显示出可靠性和适用性，在乳腺癌的早期诊断中具有很大的应用潜力。(C)2016爱思唯尔B. V.保留所有权利。
Recently, many strategies have been developed to trace the breast cancer susceptibility gene (BRCA), which is closely related to the occurrence of breast cancer. In this work, a novel recyclable electrochemical sensing platform was constructed to detect BRCA DNA based on the homogeneous hybridization of this target sequence with ferrocene-labeled DNA/Au nanospheres (FcNS) and horseradish peroxidase-labeled DNA/Au nanospheres (HRPNS) concatamers, and the host-guest interaction between cucurbit [7]uril (CB [7]) adsorbed on the electrode and Fc on the hybridization complex. With the optimization of experimental conditions, the fabricated sensing platform showed improved sensitivity with a low detection limit of 25 pM (S/N = 3), contributed by the.dual amplification of signal using FcNS and HRPNS concatamers, and high specificity for BRCA DNA. The captured complex was dissociated from CB [7]-modified electrode with the rise of pH value to recycle the sensing platform. Furthermore, this detection strategy displayed the reliability and applicability in the analysis of human serum samples, indicating great potential for applications in early diagnosis of breast cancer. (C) 2016 Elsevier B.V. All rights reserved.