Magnetic micro-device for manipulating PC12 cell migration and organization
Magnetic micro-device for manipulating PC12 cell migration and organization
复制标题
DOI：
10.1039/c5lc00035a
复制
发表时间：
2015-01-01
期刊：
影响因子：
6.1
通讯作者：
Shefi, O.
中科院分区：
文献类型：
--
作者：
Alon, N.;Havdala, T.;Shefi, O.
指导神经元的迁移和生长对潜在的创伤后治疗具有重要影响。磁操作是远程引导细胞的一种有利方法。在本研究中，我们产生了具有可控磁通密度的高度局域磁场，以在微观水平上操纵神经元样细胞的迁移和组织。我们设计并制作了一种独特的小型化磁性器件，该器件由坡莫合金(Ni80Fe20)矩形铁磁棒阵列组成，溅射沉积在玻璃衬底上。磁铁的不对称形状使人们能够在两极设计出具有高磁通密度的磁场景观。将氧化铁纳米颗粒引入PC12细胞，使细胞具有磁敏特性。首先，我们通过施加外部磁场来操纵细胞。磁场力足够强，足以引导PC12细胞在培养中迁移。基于时间推移的观察，我们分析了细胞的运动，并估计了每个细胞的MNPs数量。我们将上传的细胞电镀在微图案化的磁性设备上。这些细胞向高磁通区迁移并聚集在有图案的磁铁的边缘，证实了带有磁性纳米颗粒的细胞确实受到微磁铁的影响，并被磁条的磁极所吸引。我们的研究提出了一种新的方法，用于产生预先编程的磁性微‘热点’来定位和指导细胞生长，为植入的磁性设备奠定了基础。
Directing neuronal migration and growth has an important impact on potential post traumatic therapies. Magnetic manipulation is an advantageous method for remotely guiding cells. In the present study, we have generated highly localized magnetic fields with controllable magnetic flux densities to manipulate neuron-like cell migration and organization at the microscale level. We designed and fabricated a unique miniaturized magnetic device composed of an array of rectangular ferromagnetic bars made of permalloy (Ni80Fe20), sputter-deposited onto glass substrates. The asymmetric shape of the magnets enables one to design a magnetic landscape with high flux densities at the poles. Iron oxide nanoparticles were introduced into PC12 cells, making the cells magnetically sensitive. First, we manipulated the cells by applying an external magnetic field. The magnetic force was strong enough to direct PC12 cell migration in culture. Based on time lapse observations, we analysed the movement of the cells and estimated the amount of MNPs per cell. We plated the uploaded cells on the micro-patterned magnetic device. The cells migrated towards the high magnetic flux zones and aggregated at the edges of the patterned magnets, corroborating that the cells with magnetic nanoparticles are indeed affected by the micro-magnets and attracted to the bars' magnetic poles. Our study presents an emerging method for the generation of pre-programmed magnetic micro-'hot spots' to locate and direct cellular growth, setting the stage for implanted magnetic devices.